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ATP5A Antibody - BSA Free

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Western Blot: ATP5A Antibody [NBP2-92928] - Analysis of extracts of various cell lines, using ATP5A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: ATP5A Antibody [NBP2-92928] - Confocal immunofluorescence analysis of U-2OS cells using ATP5A antibody (NBP2-92928) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ATP5A Antibody [NBP2-92928] - Confocal immunofluorescence analysis of Hela cells using ATP5A antibody (NBP2-92928) at dilution of 1:200. Blue: DAPI for nuclear staining.
Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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ATP5A Antibody - BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1). MLSVRVAAAVVRALPRRAGLVSRNALGSSFIAARNFHASNTHLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATAS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5F1A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Simple Western Validated from a verified customer review.
  • Western Blot 1:500 - 1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-92928 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for ATP5A Antibody - BSA Free

  • ATP synthase alpha chain, mitochondrial
  • ATP synthase subunit alpha, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F1 co
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
  • ATP sythase (F1-ATPase) alpha subunit
  • ATP5A
  • ATP5A1
  • ATP5AL2
  • ATP5AMOM2
  • ATPM
  • ATPMATP synthase subunit alpha, mitochondrial
  • cardiac muscle
  • COXPD22
  • EC 3.6.3
  • EC 3.6.3.14
  • epididymis secretory sperm binding protein Li 123m
  • hATP1
  • HEL-S-123m
  • MC5DN4
  • MC5DN4A
  • MC5DN4B
  • mitochondrial ATP synthetase, oligomycin-resistant
  • MOM2
  • OMR
  • ORM
  • ORMATP synthase alpha chain, mitochondrial

Background

ATP5A encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP5A Antibody (NBP2-92928) (0)

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Review for ATP5A Antibody (NBP2-92928) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-92928:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
Simple Western Human 04/06/2023
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Summary

ApplicationSimple Western
Sample Testedhuman brain lysate
SpeciesHuman
Lot0025060101

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP5A Antibody (NBP2-92928) (0)

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Secondary Antibodies

 

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Verified Customer
04/06/2023
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol ATP5F1A