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ASCL3 Antibody

Images

 
Western Blot: ASCL3 Antibody [NBP1-68871] - (0.1ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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ASCL3 Antibody Summary

Immunogen
Peptide with sequence C-HLPEDYLEKRLSK corresponding to internal region according to Uniprot Mouse NP_064435.1.
Epitope
C-HLPEDYLEKRLSK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ASCL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.1-0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA detection limit 1:8000
  • Western Blot 0.1 ug/ml
Application Notes
Western blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).
Publications
Read Publication using NBP1-68871.

Reactivity Notes

Expected from sequence similarity: Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASCL3 Antibody

  • achaete-scute complex homolog 3 (Drosophila)
  • ASH-3
  • bHLH transcription factor Sgn-1 (Salivary Glands 1)
  • bHLH transcriptional regulator Sgn-1
  • BHLHA42
  • bHLHa42achaete-scute homolog 3
  • Class A basic helix-loop-helix protein 42
  • hASH3
  • HASH3achaete-scute complex (Drosophila) homolog-like 3
  • Sgn1

Background

Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASCL3 Antibody (NBP1-68871)(1)

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Bioinformatics

Gene Symbol ASCL3
Entrez
Uniprot