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alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted

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Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted [NBP2-48130] - Formalin-fixed, paraffin-embedded human prostate carcinoma (20X) stained with AMACR / p504S Rabbit PAb.
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted [NBP2-48130] - Formalin-fixed, paraffin-embedded human prostate carcinoma (20X) stained with AMACR / p504S Rabbit PAb.
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted [NBP2-48130] - Human Prostate Carcinoma stained with AMACR / p504S Rabbit Polyclonal Antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32916-0.1mg
Azide and BSA Free
NBP2-34514-0.1mg
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alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted Summary

Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Immunogen
A synthetic peptide from human alpha-Methylacyl-CoA Racemase/AMACR protein (Uniprot: Q9UHK6)
Localization
Cytoplasmic
Marker
Prostate Cancer Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMACR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed): 1:50-1:100 for 30-60 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody - IHC-Prediluted

  • 2-methylacyl-CoA racemase
  • alpha-Methylacyl-CoA Racemase
  • alphaMethylacylCoARacemase
  • AMACR
  • AMACRD
  • CBAS4
  • EC 5.1.99.4
  • RACE
  • RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AMACR