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alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)

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Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody ...read more
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) [NBP2-53390] - Western Blot Analysis of Liver tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
AMACR/1723
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54501AF350
Alexa Fluor 405
NBP2-54501AF405
Alexa Fluor 488
NBP2-54501AF488
Alexa Fluor 532
NBP2-54501AF532
Alexa Fluor 594
NBP2-54501AF594
Alexa Fluor 647
NBP2-54501AF647
Alexa Fluor 700
NBP2-54501AF700
Alexa Fluor 750
NBP2-54501AF750
Allophycocyanin
NBP2-54501APC
Biotin
NBP2-54501B
CoraFluor 1
NBP2-54501CL1
DyLight 350
NBP2-54501UV
DyLight 405
NBP2-54501V
DyLight 488
NBP2-54501G
DyLight 550
NBP2-54501R
DyLight 594
NBP2-54501DL594
DyLight 650
NBP2-54501C
DyLight 680
NBP2-54501FR
DyLight 755
NBP2-54501IR
FITC
NBP2-54501F
HRP
NBP2-54501H
Janelia Fluor 525
NBP2-54501JF525
Janelia Fluor 549
NBP2-54501JF549
Janelia Fluor 585
NBP2-54501JF585
Janelia Fluor 635
NBP2-54501JF635
Janelia Fluor 646
NBP2-54501JF646
Janelia Fluor 669
NBP2-54501JF669
PE
NBP2-54501PE
PerCP
NBP2-54501PCP
Unconjugated
NBP2-54501-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-54501-100ug
Azide and BSA Free
NBP2-54501-100ug
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alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54501)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human alpha-Methylacyl-CoA Racemase/AMACR protein (Uniprot: Q9UHK6)
Localization
Cytoplasmic
Marker
Prostate Cancer Marker
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
AMACR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)

  • 2-methylacyl-CoA racemase
  • alpha-Methylacyl-CoA Racemase
  • alphaMethylacylCoARacemase
  • AMACR
  • AMACRD
  • CBAS4
  • EC 5.1.99.4
  • RACE
  • RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AMACR
Uniprot