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ALDH4A1 Antibody

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Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Analysis in human kidney and lymph node tissues using NBP1-89149 antibody. Corresponding ALDH4A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human kidney shows high expression.
Western Blot: ALDH4A1 Antibody [NBP1-89149] -Analysis in human kidney tissue.
Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ALDH4A1 Antibody [NBP1-89149] - Staining of human lymph node shows no positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ALDH4A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLE
Predicted Species
Mouse (91%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ALDH4A1 Protein (NBP1-89149PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ALDH4A1 Antibody

  • aldehyde dehydrogenase 4 family, member A1
  • Aldehyde dehydrogenase family 4 member A1
  • ALDH4DKFZp779M035
  • delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
  • EC 1.5.1.12
  • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase
  • P5C dehydrogenase
  • P5CD
  • P5CDH

Background

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ALDH4A1
Entrez
Uniprot