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AKT2 Antibody (OTI4H7) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI4H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70146AF350
Alexa Fluor 405
NBP2-70146AF405
Alexa Fluor 488
NBP2-70146AF488
Alexa Fluor 532
NBP2-70146AF532
Alexa Fluor 594
NBP2-70146AF594
Alexa Fluor 647
NBP2-70146AF647
Alexa Fluor 700
NBP2-70146AF700
Alexa Fluor 750
NBP2-70146AF750
Allophycocyanin
NBP2-70146APC
Allophycocyanin/Cy7
NBP2-70146APCCY7
Biotin
NBP2-70146B
CoraFluor 1
NBP2-70146CL1
DyLight 350
NBP2-70146UV
DyLight 405
NBP2-70146V
DyLight 488
NBP2-70146G
DyLight 550
NBP2-70146R
DyLight 594
NBP2-70146DL594
DyLight 650
NBP2-70146C
DyLight 680
NBP2-70146FR
DyLight 755
NBP2-70146IR
FITC
NBP2-70146F
HRP
NBP2-70146H
Janelia Fluor 525
NBP2-70146JF525
Janelia Fluor 549
NBP2-70146JF549
Janelia Fluor 585
NBP2-70146JF585
Janelia Fluor 646
NBP2-70146JF646
Janelia Fluor 669
NBP2-70146JF669
PE
NBP2-70146PE
PE/Atto594
NBP2-70146PEATT594
PE/Cy5.5
NBP2-70146PECY55
PE/Cy7
NBP2-70146PECY7
PerCP
NBP2-70146PCP
Unconjugated
NBP2-02231
mFluor Violet 450 SE
NBP2-70146MFV450
mFluor Violet 500 SE
NBP2-70146MFV500
mFluor Violet 610 SE
NBP2-70146MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02231
Azide and BSA Free
NBP2-70146
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AKT2 Antibody (OTI4H7) [Janelia Fluor® 635] Summary

Immunogen
Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AKT2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for AKT2 Antibody (OTI4H7) [Janelia Fluor® 635]

  • Akt2
  • EC 2.7.11
  • EC 2.7.11.1
  • Murine thymoma viral (v-akt) homolog-2
  • PKB beta
  • PKBB
  • PKBBETA
  • PRKBB
  • Protein kinase Akt-2
  • Protein kinase B beta
  • rac protein kinase beta
  • RAC-beta serine/threonine-protein kinase
  • RAC-beta
  • RAC-PK-beta
  • v-akt murine thymoma viral oncogene homolog 2

Background

AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AKT2 Antibody
(OTI4H7) [Janelia Flu ...
NBP2-70146JF635
AKT2 Antibody (OTI4H7) [Janelia Fluor® 635]
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKT2