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Adenine Nucleotide Translocator 2 Antibody

Images

 
Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-56762] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocator 2 Antibody [NBP2-56762] - Staining of human cell line PC-3 shows localization to mitochondria.
Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-56762] - Analysis in human testis tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Adenine Nucleotide Translocator 2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AAVSKTAVAPIERVKLLLQVQHASKQIS
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC25A5
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Adenine Nucleotide Translocator 2 Recombinant Protein Antigen (NBP2-56762PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Adenine Nucleotide Translocator 2 Antibody

  • adenine nucleotide translocator 2 (fibroblast)
  • Adenine nucleotide translocator 2
  • ADP
  • ADP/ATP carrier protein
  • ADP/ATP translocase 2
  • ANT2AAC2, 2F1
  • ATP carrier protein 2
  • ATP carrier protein, fibroblast isoform
  • solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 5
  • Solute carrier family 25 member 5
  • T2
  • T3ANT 2

Background

ADP/ATP translocase, the most abundant mitochondrial protein, is an integral component of the inner mitochondrial membrane. It facilitates exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartment of ATP production to those of ATP utilization. SLC25A5 is 1 of at least 3 transcriptionally active ADP/ATP translocase genes in humans (Chen et al., 1990 [PubMed 2157297]). Battini et al. (1987) [PubMed 3031073] cloned an ADP/ATP translocase gene from an Okayama-Berg library derived from SV40-transformed human fibroblasts. Ku et al. (1990) [PubMed 2168878] cloned and sequenced the ANT2 gene. Chen et al. (1990) [PubMed 2157297] isolated 7 ADP/ATP translocase pseudogenes from recombinant human genomic libraries. Each pseudogene sequence had more than 85% identity with the sequence of the translocase cDNA derived from fibroblast mRNA, but each had mutations that precluded synthesis of a functional protein.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC25A5