Home » 14-3-3 zeta » 14-3-3 zeta Lysates » 14-3-3 zeta Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

14-3-3 zeta Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: 14-3-3 zeta Overexpression Lysate (Adult Normal) [NBL1-17952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta.
Western Blot: 14-3-3 zeta Overexpression Lysate (Adult Normal) [NBL1-17952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 zeta.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

14-3-3 zeta Overexpression Lysate Summary

Description

14-3-3 zeta Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209909

Accession#: NM_003406

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
YWHAZ

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for 14-3-3 zeta Overexpression Lysate

  • 1433 zeta
  • 14-3-3 zeta
  • KCIP-1
  • KCIP-1MGC126532,14-3-3-zeta
  • MGC111427
  • MGC138156
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
  • YWHAZ
  • zeta polypeptide

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

PLA2G2A Antibody
[Unconjugated]
AF4925
Western Blot PLA2G2A Antibody [Unconjugated]
Immunohistochemistry PLA2G2A Antibody [Unconjugated]
Simple Western PLA2G2A Antibody [Unconjugated]
Species: Mu
Applications: IHC, Simple Western, WB
PLA2G6 Antibody -
Azide and BSA Free
H00008398-D01P
Western Blot PLA2G6 Antibody - Azide and BSA Free
Western Blot PLA2G6 Antibody - Azide and BSA Free
Western Blot PLA2G6 Antibody - Azide and BSA Free
Species: Hu, Mu
Applications: WB
PLA2G1B Antibody
(630823)
MAB5018
Western Blot PLA2G1B Antibody (630823)
Species: Hu
Applications: IP, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(822 Publications)
Species: Hu
Applications: BA
TEAD1 Antibody -
Azide and BSA Free
NBP2-94035
Chromatin Immunoprecipitation TEAD1 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence TEAD1 Antibody - Azide and BSA Free
Western Blot TEAD1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
DBT Antibody - BSA
Free
NBP1-89522
Immunocytochemistry/ Immunofluorescence DBT Antibody - BSA Free
Immunohistochemistry-Paraffin DBT Antibody - BSA Free
Immunohistochemistry-Paraffin DBT Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PLA2G4A Antibody -
BSA Free
NBP1-31344
Immunocytochemistry/ Immunofluorescence PLA2G4A Antibody - BSA Free
Immunohistochemistry-Paraffin PLA2G4A Antibody - BSA Free
Western Blot PLA2G4A Antibody - BSA Free
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
PRRT2 Antibody -
BSA Free
NBP2-82026
Western Blot PRRT2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PRRT2 Antibody - BSA Free
Immunohistochemistry PRRT2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(55 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
IL-1 beta/IL-1F2
[Unconjugated]
201-LB
SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]
Bioactivity IL-1 beta/IL-1F2 [Unconjugated]
SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]
(601 Publications)
Species: Hu
Applications: BA
Kininogen Antibody
- BSA Free
NBP1-89993
Immunohistochemistry-Paraffin Kininogen Antibody - BSA Free
Immunohistochemistry-Paraffin Kininogen Antibody - BSA Free
Western Blot Kininogen Antibody - BSA Free
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Phospholipase B1
Antibody (C4)
NBP3-41952
Immunohistochemistry-Paraffin Phospholipase B1 Antibody (C4)
Species: Hu, Po
Applications: ICC/IF, IHC, WB
TIP60 Antibody -
BSA Free
NBP2-24613
Immunohistochemistry-Paraffin TIP60 Antibody - BSA Free
Western Blot TIP60 Antibody - BSA Free
Simple Western TIP60 Antibody - BSA Free
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(676 Publications)
Species: Mu
Applications: ELISA
5-Lipoxygenase
Antibody - BSA Free
NB110-58748
Western Blot 5-Lipoxygenase Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence 5-Lipoxygenase Antibody - BSA Free
Simple Western 5-Lipoxygenase Antibody - BSA Free
(2 Reviews)
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, KD, Simple Western, WB
COX-2 Antibody
NB100-689
Immunohistochemistry-Paraffin COX-2 Antibody
Simple Western COX-2 Antibody
(1 Review)
(36 Publications)
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB

Endorepellin/Perlecan/ ...
AF2364
Immunohistochemistry Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]
(6 Publications)
Species: Hu
Applications: IHC, WB
14-3-3 zeta
Overexpression Lysate
NBL1-17952
14-3-3 zeta Overexpression Lysate
Species: Hu
Applications: WB

Publications for 14-3-3 zeta Lysate (NBL1-17952) (0)

There are no publications for 14-3-3 zeta Lysate (NBL1-17952).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 14-3-3 zeta Lysate (NBL1-17952) (0)

There are no reviews for 14-3-3 zeta Lysate (NBL1-17952). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 14-3-3 zeta Lysate (NBL1-17952) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional 14-3-3 zeta Products

Research Areas for 14-3-3 zeta Lysate (NBL1-17952)

Find related products by research area.

Blogs on 14-3-3 zeta

There are no specific blogs for 14-3-3 zeta, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
14-3-3 zeta Antibody (818515) [Unconjugated]
MAB2669

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 14-3-3 zeta Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol YWHAZ