Sort By:

Products (32)

page of 3

N-Cadherin Antibodies

N-Cadherin, also referred to as Neural Cadherin (NCAD) or Cadherin-2 (CHD2), is a member of the calcium-dependent adhesion molecule family of classical (type I) cadherins. N-cadherin is most highly expressed by mesenchymal cells and neural tissue, and functions in maintaining structural integrity and adhesion, cell signaling, and synapse formation. Human N-cadherin is synthesized as a 906 amino acid protein with a theoretical molecular weight of 99.8 kDa. Browse our catalog for recombinant antibodies, monoclonal and polyclonal antibodies, and N-Cadherin-conjugated antibodies. Common research applications for our N-Cadherin antibodies include CyTOF-Ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Simple Western, and both Knockdown and Knockout Validated Western Blot. Our N-Cadherin antibodies are suitable for use in many species: Human, Mouse, Rat, Bovine, Canine, and Chicken to name a few. Each N-Cadherin antibody is fully covered by Novus’ Guarantee+. Choose the anti-N-Cadherin antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.

Alternate Names for N-Cadherin Antibodies

anti-N-Cadherin antibody, anti-CDH2 antibody, anti-cadherin 2, N-cadherin (neuronal) antibody, anti-cadherin 2, type 1, N-cadherin (neuronal) antibody, anti-cadherin-2 antibody, anti-CD325 antibody, anti-CD325 antigen antibody, anti-CDHNcalcium-dependent adhesion protein, neuronal antibody, anti-CDw325 antibody, anti-NCADN-cadherin 1 antibody, anti-Neural cadherin antibody, anti-neural-cadherin antibody

32 Results for "n-cadherin" in Primary Antibodies

List

Grid

NBP1-48309

N-Cadherin Antibody (13A9)

(2 Reviews)

(41 Publications)

0.1 ml

NBP1-48309

Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.

Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.

Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.

Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-48309AF350
Alexa Fluor 405	NBP1-48309AF405
Alexa Fluor 488	NBP1-48309AF488
Alexa Fluor 532	NBP1-48309AF532
Alexa Fluor 594	NBP1-48309AF594
Alexa Fluor 647	NBP1-48309AF647
Alexa Fluor 700	NBP1-48309AF700
Alexa Fluor 750	NBP1-48309AF750
Allophycocyanin/Cy7	NBP1-48309APCCY7
Biotin	NBP1-48309B
CoraFluor 1	NBP1-48309CL1
DyLight 350	NBP1-48309UV
DyLight 405	NBP1-48309V
DyLight 488	NBP1-48309G
DyLight 550	NBP1-48309R
DyLight 594	NBP1-48309DL594
DyLight 650	NBP1-48309C
DyLight 680	NBP1-48309FR
DyLight 755	NBP1-48309IR
FITC	NBP1-48309F
HRP	NBP1-48309H
Janelia Fluor 525	NBP1-48309JF525
Janelia Fluor 549	NBP1-48309JF549
Janelia Fluor 585	NBP1-48309JF585
Janelia Fluor 635	NBP1-48309JF635
Janelia Fluor 646	NBP1-48309JF646
Janelia Fluor 669	NBP1-48309JF669
PE/Atto594	NBP1-48309PEATT594
PE/Cy5.5	NBP1-48309PECY55
PE/Cy7	NBP1-48309PECY7
mFluor Violet 450 SE	NBP1-48309MFV450
mFluor Violet 500 SE	NBP1-48309MFV500
mFluor Violet 610 SE	NBP1-48309MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80868

compare

(select up to 3 products from this page to compare)

AF6426

N-Cadherin Antibody [Unconjugated]

(24 Publications)

100 μg

AF6426

Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Human N‑Cadherin protein was serially diluted 2-fold and captured by Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

A172 cells were stained with Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Fluorescein-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0125). View our protocol for Staining Membrane-associated Proteins.

N-Cadherin Depletion Rescues the Migration Defect in Rab14- and FAM116-Depleted Cells(A and B) A549 cells were treated with control, Rab14, FAM116A, N-cadherin, E-cadherin, and FGFR2 siRNA duplexes alone or in the combinations indicated for 72 hr (A). The cells were then western blotted for N-cadherin, E-cadherin, or tubulin as a loading control or (B) fixed and then stained with DAPI and antibodies to N-cadherin. Scale bar indicates 10 μm.(C) A549 cells were treated with control, Rab14, FAM116A, N-cadherin, E-cadherin, and FGFR2 siRNA duplexes alone or in the combinations indicated for 72 hr. The cell monolayers were then scratched and imaged for 16 hr. Cell migration was measured and is plotted on the graph with error bars to show the standard error of the mean (n = 3).(D) Images are shown from the 0 and 16 hr time points for the Rab14- and FAM116A-depleted cells in the presence and absence of N-cadherin siRNA. Scale bar indicates 50 μm.See also Figure S5. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1534580712001876), licensed under a CC-BY license. Not internally tested by R&D Systems.

Abnormal Adherens Junctions in Rab14- and FAM116-Depleted Cells(A) A549 cells were treated with control, Rab4, Rab11, Rab14, FAM116A, and Avl9 siRNA duplexes for 72 hr. The cells were lysed in SDS-PAGE sample buffer and then western blotted as indicated in the figure. Tubulin was used as a loading control.(B) A549 cells were treated with control, Rab14, and FAM116A siRNA duplexes for 72 hr, fixed, and then stained with DAPI and antibodies to N-cadherin.(C) A549 cells were treated with control, Rab4, Rab11, Rab14, FAM116A, and Avl9 siRNA duplexes for 72 hr. The cell monolayers were then scratched, samples fixed at 0, 4, 8, and 16 hr, and then stained with DAPI and antibodies to N-cadherin. Images are oriented so the wound is to the right. Scale bar indicates 10 μm in all images.See also Figure S6. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1534580712001876), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	FAB6426G
Allophycocyanin	FAB6426A

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB6426A
Carrier Free	FAB6426G
Carrier Free	AF6426-SP

compare

(select up to 3 products from this page to compare)

NBP1-51612

N-Cadherin Antibody (5D5) - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NBP1-51612

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (5D5) [NBP1-51612] - Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human Placenta tissues using CDH2 mouse mAb

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-51612-0.025ml

compare

(select up to 3 products from this page to compare)

NBP2-01498

N-Cadherin Antibody (OTI2G7)

(1 Publication)

0.1 ml

NBP2-01498

Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.

Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.

Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72891AF350
Alexa Fluor 405	NBP2-72891AF405
Alexa Fluor 488	NBP2-72891AF488
Alexa Fluor 532	NBP2-72891AF532
Alexa Fluor 594	NBP2-72891AF594
Alexa Fluor 647	NBP2-72891AF647
Alexa Fluor 700	NBP2-72891AF700
Alexa Fluor 750	NBP2-72891AF750
Allophycocyanin	NBP2-72891APC
Allophycocyanin/Cy7	NBP2-72891APCCY7
Biotin	NBP2-72891B
CoraFluor 1	NBP2-72891CL1
DyLight 350	NBP2-72891UV
DyLight 405	NBP2-72891V
DyLight 488	NBP2-72891G
DyLight 550	NBP2-72891R
DyLight 594	NBP2-72891DL594
DyLight 650	NBP2-72891C
DyLight 680	NBP2-72891FR
DyLight 755	NBP2-72891IR
FITC	NBP2-72891F
HRP	NBP2-72891H
Janelia Fluor 525	NBP2-72891JF525
Janelia Fluor 549	NBP2-72891JF549
Janelia Fluor 585	NBP2-72891JF585
Janelia Fluor 635	NBP2-72891JF635
Janelia Fluor 646	NBP2-72891JF646
Janelia Fluor 669	NBP2-72891JF669
PE	NBP2-72891PE
PE/Atto594	NBP2-72891PEATT594
PE/Cy5.5	NBP2-72891PECY55
PE/Cy7	NBP2-72891PECY7
PerCP	NBP2-72891PCP
mFluor Violet 450 SE	NBP2-72891MFV450
mFluor Violet 500 SE	NBP2-72891MFV500
mFluor Violet 610 SE	NBP2-72891MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72891

compare

(select up to 3 products from this page to compare)

NBP2-19457

N-Cadherin Antibody

(1 Publication)

0.1 ml

NBP2-19457

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: N-Cadherin Antibody [NBP2-19457] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody [NBP2-19457] - SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: N-Cadherin Antibody [NBP2-19457] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-19459

N-Cadherin Antibody

(1 Publication)

0.1 ml

NBP2-19459

Western Blot: N-Cadherin Antibody [NBP2-19459] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody ( diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 5% SDS-PAGE N-Cadherin antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-38856

N-Cadherin Antibody

(4 Publications)

0.1 ml

NBP2-38856

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human skin shows no positivity in keratinocytes.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-61632

N-Cadherin Antibody (CL3716)

100 ul

NBP2-61632

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining in human heart muscle and skin tissues. Corresponding CDH2 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of U-251 cells using the Anti-CDH2 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251 MG

Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human cerebral cortex shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human skin shows no positivity in epidermal cells as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-19460

N-Cadherin Antibody

0.1 ml

NBP2-19460

Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19460] - Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal ) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: N-Cadherin Antibody [NBP2-19460] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal diluted at 1:1000.

Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.

Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP3-15654

N-Cadherin Antibody (6F9G8)

100 ul

NBP3-15654

Immunohistochemistry-Paraffin: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Rat heart using N-Cadherin Rabbit mAb (NBP3-15654) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Mouse heart using N-Cadherin Rabbit mAb (NBP3-15654) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

<b>Genetic Strategies Validation. </b>Knockout Validated: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

AF1388

N-Cadherin Antibody [Unconjugated] - Propeptide

(1 Publication)

100 μg

AF1388

Western blot shows lysates of IMR-32 human neuroblastoma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human N-Cadherin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Pro-N-Cadherin was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1388

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1388-SP

compare

(select up to 3 products from this page to compare)

NBP2-53321

N-Cadherin Antibody (8C11)

(2 Publications)

100 μg

NBP2-53321

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54523AF350
Alexa Fluor 405	NBP2-54523AF405
Alexa Fluor 488	NBP2-54523AF488
Alexa Fluor 532	NBP2-54523AF532
Alexa Fluor 594	NBP2-54523AF594
Alexa Fluor 647	NBP2-54523AF647
Alexa Fluor 700	NBP2-54523AF700
Alexa Fluor 750	NBP2-54523AF750
Allophycocyanin	NBP2-54523APC
Allophycocyanin/Cy7	NBP2-54523APCCY7
Biotin	NBP2-54523B
CoraFluor 1	NBP2-54523CL1
DyLight 350	NBP2-54523UV
DyLight 405	NBP2-54523V
DyLight 488	NBP2-54523G
DyLight 550	NBP2-54523R
DyLight 594	NBP2-54523DL594
DyLight 650	NBP2-54523C
DyLight 680	NBP2-54523FR
DyLight 755	NBP2-54523IR
FITC	NBP2-54523F
HRP	NBP2-54523H
Janelia Fluor 525	NBP2-54523JF525
Janelia Fluor 549	NBP2-54523JF549
Janelia Fluor 585	NBP2-54523JF585
Janelia Fluor 635	NBP2-54523JF635
Janelia Fluor 646	NBP2-54523JF646
Janelia Fluor 669	NBP2-54523JF669
PE	NBP2-54523PE
PE/Atto594	NBP2-54523PEATT594
PE/Cy5.5	NBP2-54523PECY55
PE/Cy7	NBP2-54523PECY7
PerCP	NBP2-54523PCP
mFluor Violet 450 SE	NBP2-54523MFV450
mFluor Violet 500 SE	NBP2-54523MFV500
mFluor Violet 610 SE	NBP2-54523MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54523-100ug

compare

(select up to 3 products from this page to compare)

page of 3

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

N-Cadherin Antibodies

N-Cadherin Antibody (13A9)

N-Cadherin Antibody [Unconjugated]

N-Cadherin Antibody (5D5) - BSA Free

N-Cadherin Antibody (OTI2G7)

N-Cadherin Antibody

N-Cadherin Antibody

N-Cadherin Antibody

N-Cadherin Antibody (CL3716)

N-Cadherin Antibody

N-Cadherin Antibody (6F9G8)

N-Cadherin Antibody [Unconjugated] - Propeptide

N-Cadherin Antibody (8C11)