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Heart Induction Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Heart Induction Pathway and Ectopia Cordis, Neuroblastoma, Myocardial Infarction, Tumor Angiogenesis, Overlying Behavior. The study of the Heart Induction Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Heart Induction Pathway has been researched in relation to Heart Development, Gastrulation, Heart Formation, Regeneration, Fertilization. The Heart Induction Pathway complements our catalog of research reagents including antibodies and ELISA kits against WNT, DICKKOPF-1, CHORDIN, ACVR1B, BMP4.

Top Research Reagents

We have 1474 products for the study of the Heart Induction Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Activin RIB/ALK-4 Antibody (3U6I8)
NBP3-16106
Western Blot: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Western blot analysis of extracts of various cell lines, using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Immunohistochemistry of paraffin-embedded mouse brain using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

CRIPTO Antibody - BSA Free
NB100-1598
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

3 Publications
GSK-3 beta Antibody (3D10) - BSA Free
NBP1-47470
Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     4 Reviews

10 Publications
Myosin Light Chain 2 Antibody
NBP1-85541
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart muscle and pancreas tissues. Corresponding Myosin Light Chain 2 RNA-seq data are presented for the same tissues.Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart tissue.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-Fr

     1 Review

2 Publications
preproANP Antibody
NBP2-14873
Western Blot: preproANP Antibody [NBP2-14873] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

6 Publications
SS18L1 Antibody
NBP2-20486
Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

Cerberus 1 Antibody [Unconjugated]
AF1075
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB

     1 Review

6 Publications
beta-Catenin Antibody [Unconjugated]
AF1329
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta ‑Catenin/<BR>DNA complexes were immuno­precipitated using 5 μg Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     2 Reviews

33 Publications
NKX2.5 Antibody [Unconjugated]
AF2444
EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating doxycycline-dependent EOMES expression in a transgenic EOMESKO/E.TET-ON hESC line. b Typical yields of hESC-CMs (left, flow cytometry) and NKX2.5 expression (right, immunoblot) obtained with the two protocols (day 10). c Immunostainings 21 days after the initiation of EOMES induction. Weak perinuclear ANP staining is typical in overall MLC2v-positive hESC-CMs. Scale bars: 25 (top) and 50 µm (bottom). d Acceleration and slowdown of spontaneous beat rates in pCMs following exposure to 10 µM isoprenaline and 10 µM propranolol, respectively, on multielectrode arrays. e Microarray-based time course analysis comparing the indicated protocols and cell lines. RESCUE cells carry an inducible EOMES transgene on EOMESKO HuES6 background. Underlying data are from Supplementary Data 2 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.Contextual requirements of EOMES-mediated CM programming. a DOX dose dependency of the EOMES TET-ON protocol using the WTE.TET-ON hESC line. Top panel: Immunostains at 1.5 wk. Scale bar: 100 µm. Bottom: Flow cytometry analysis. Numbers indicate average percentages of cTnT-positive CMs from 3–6 experiments per condition. b CM programming by EOMES necessitates suppression of autocrine WNT signaling from the third day of transgene induction (qPCR data, n = 2). c DOX dose-dependent CM programming using an independent WTE.TET-ON hiPSC line. The data shows immunostaining (scale bar: 100 µm), RT-qPCR (n = 4), and FACS analysis (n = 3) performed at 1 wk of differentiation. The asymmetrical shape of the DOX titration data with this line is in part due to the incomplete repression of SOX2 at 0.05 µg/ml, which caused overgrowth of the cultures with neural precursors (also see Supplementary Fig. 3f). Error bars: s.e.m. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Human
Applications WB, IHC

     2 Reviews

13 Publications
Wnt-8a Antibody [Unconjugated]
AF2248

Goat Polyclonal
Species Mouse
Applications WB, IHC

3 Publications
TCF7L1/TCF3 Antibody
AF6116
Western blot shows lysates of human pancreas and human lung tissue. PVDF Membrane was probed with 0.2 µg/mL of Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=TCF7L1 was detected in immersion fixed BG01V human embryonic stem cells using Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # <a class=

Sheep Polyclonal
Species Human
Applications WB, ICC

2 Publications
Wnt-11 [Unconjugated]
6179-WN


Species Human
Applications BA

20 Publications
Dkk-1 [Unconjugated]
5439-DK
Recombinant Human Dkk‑1 (Catalog # 5439-DK) inhibits Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 10.0-250 ng/mL.JOY6 human iPSCs were cultured in media containing Cultrex™ Stem Cell Qualified RGF BME (<a class=


Species Human
Applications BA

90 Publications
Nodal [Unconjugated]
3218-ND


Species Human
Applications BA

22 Publications
Wnt-5a [Unconjugated]
645-WN
Recombinant mouse Wnt-5a (<a class=NoLineLink href=


Species Human, Mouse
Applications BA

180 Publications
BMP-4 [Unconjugated]
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

519 Publications
HHEX Antibody (2018B) [Unconjugated]
MAB83771
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat HHEX Monoclonal Antibody (Catalog # MAB83771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=HHEX was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Rabbit Anti-Human/Mouse/Rat HHEX Polyclonal Antibody (Catalog # MAB83771) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC

6 Publications