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![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining in human bone marrow and liver tissues . Corresponding NFE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining in human bone marrow and liver tissues . Corresponding NFE2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in control (vector only transfected HEK293T lysate) and NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0019.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0018.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human bone marrow shows moderate to strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0014.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0015.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0016.jpg)
![Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Simple Western lane view shows a specific band for NFE2 in 0.2 mg/ml of K-562 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Simple-Western-NBP1-82580-img0006.jpg)
![Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Electropherogram image(s) of corresponding Simple Western lane view. Nuclear Factor Erythroid Derived 2 antibody was used at 1:20 dilution on K-562 lysate(s).](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Simple-Western-NBP1-82580-img0007.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Western blot analysis of Nuclear Factor Erythroid Derived 2 on different lysates with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. Lane 1: U-937 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: TF-1 cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: Mouse testis tissue lysate (30ug/Lane) Lane 7: Rat testis tissue lysate (30ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-27820241524264.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Western blot analysis of Nuclear Factor Erythroid Derived 2 on different lysates with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. Lane 1: U-937 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: TF-1 cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: Mouse testis tissue lysate (30ug/Lane) Lane 7: Rat testis tissue lysate (30ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32646) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-298202414115440.jpg)
![Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunocytochemistry analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2 with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-29202410234219.jpg)
![Flow Cytometry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Flow cytometric analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32646, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-29202416453280.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-31540-img0007.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-E2 protein stained by NF-E2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31540-img0005.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (SR1755) [NBP3-22631] - Western blot analysis using NBP3-22631 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22631_rabbit-nuclear-factor-erythroid-derived-2-mab-sr1755-5720231524619.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (SR1755) [NBP3-22631] - Western blot analysis using NBP3-22631 in K562 cell lysate.")
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (2C6) [H00004778-M01] - Analysis of NFE2 expression in transfected 293T cell line by NFE2 monoclonal antibody (M01), clone 2C6.Lane 1: NFE2 transfected lysate(41.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-2C6-Western-Blot-H00004778-M01-img0004.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (2C6) [H00004778-M01] - Analysis of NFE2 expression in transfected 293T cell line by NFE2 monoclonal antibody (M01), clone 2C6.Lane 1: NFE2 transfected lysate(41.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Nuclear Factor Erythroid Derived 2 Antibody (2C6) [H00004778-M01] - Detection limit for recombinant GST tagged NFE2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-2C6-Sandwich-ELISA-H00004778-M01-img0003.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP3-09364] - Western blot analysis using NBP3-09364 on Human 293T as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP3-09364-img0001.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP3-09364] - Western blot analysis using NBP3-09364 on Human 293T as a positive control. Antibody Titration: 0.2-1 ug/ml")
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-69226] - Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-69226-img0004.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-69226] - Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate.")
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP3-10897] - Western blot analysis of Nuclear Factor Erythroid Derived 2 in Mouse Heart. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP3-10897-img0001.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP3-10897] - Western blot analysis of Nuclear Factor Erythroid Derived 2 in Mouse Heart. Antibody dilution at 1ug/ml")
![Western Blot: Nuclear Factor Erythroid Derived 2 Overexpression Lysate (Adult Normal) [NBP2-11086] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuclear Factor Erythroid Derived 2.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11086-img0001.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Overexpression Lysate (Adult Normal) [NBP2-11086] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuclear Factor Erythroid Derived 2.")
![Western Blot: Nuclear Factor Erythroid Derived 2 Overexpression Lysate (Adult Normal) [NBL1-13612] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuclear Factor Erythroid Derived 2.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-Derived-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13612-img0002.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Overexpression Lysate (Adult Normal) [NBL1-13612] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuclear Factor Erythroid Derived 2.")