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![Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-Western-Blot-NBP2-52477-img0004.jpg "Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)")
![Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded breast cancer tissues using GNAS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-Immunohistochemistry-NBP2-52477-img0003.jpg)
![Flow Cytometry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of A549 cells using GNAS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-Flow-Cytometry-NBP2-52477-img0001.jpg)
![Western Blot: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis using GNAS mAb against HEK293 (1) and GNAS (AA: 42-188)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-Western-Blot-NBP2-52477-img0005.jpg)
![Immunohistochemistry: G protein alpha Antibody (2A2B7) [NBP2-52477] - Analysis of paraffin-embedded lung cancer tissues using GNAS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-Immunohistochemistry-NBP2-52477-img0002.jpg)
![ELISA: G protein alpha Antibody (2A2B7) [NBP2-52477] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-2A2B7-ELISA-NBP2-52477-img0006.jpg)
![Western Blot: G protein alpha Antibody [NBP1-31730] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-31730-img0009.jpg "Western Blot: G protein alpha Antibody [NBP1-31730] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-31730] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-31730-img0002.jpg)
![Western Blot: G protein alpha Antibody [NBP1-31730] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31730_rabbit-polyclonal-g-protein-alpha-antibody-western-blot-2542024105050..jpg)
![Western Blot: G protein alpha Antibody [NBP1-31730] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31730_rabbit-polyclonal-g-protein-alpha-antibody-western-blot-2542024105211..jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: G protein alpha Antibody [NBP1-31730] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31730_rabbit-polyclonal-g-protein-alpha-antibody-1610202419414574.jpg)
![Western Blot: G protein alpha Antibody [NBP1-58349] - Sample Tissue: Human Lung Tumor Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-58349-img0008.jpg "Western Blot: G protein alpha Antibody [NBP1-58349] - Sample Tissue: Human Lung Tumor Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: G protein alpha Antibody [NBP1-58349] - Paraffin Embedded Tissue: Human Pancreas Antibody Concentration: 5 ug/ml](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-NBP1-58349-img0007.jpg)
![Western Blot: G protein alpha Antibody [NBP1-58349] - Titration: 1 ug/ml Positive Control: MCF-7 whole cell lysates.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-58349-img0003.jpg)
![Western Blot: G protein alpha Antibody [NBP1-58349] - Sample Tissue: MCF7 Whole Cell, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-58349-img0004.jpg)
![Immunohistochemistry: G protein alpha Antibody [NBP1-58349] - Analysis of human thyroid after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-NBP1-58349-img0005.jpg)
![Immunohistochemistry: G protein alpha Antibody [NBP1-58349] - Lanes: Lane 1 : INS1 lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Donkey anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Gene name: GNAS Submitted by: Olivier Costa, Diabetes research center VUB.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-NBP1-58349-img0006.jpg)
![Western Blot: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-7G6G5-Western-Blot-NBP2-52476-img0003.jpg "Western Blot: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis using GNAS mAb against human GNAS (AA: 42-188) recombinant protein. (Expected MW is 42.8 kDa)")
![Immunocytochemistry/Immunofluorescence: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis of Hela cells using GNAS mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-7G6G5-Immunofluorescence-NBP2-52476-img0002.jpg)
![Flow Cytometry: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis of MCF-7 cells using GNAS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-7G6G5-Flow-Cytometry-NBP2-52476-img0001.jpg)
![Western Blot: G protein alpha Antibody (7G6G5) [NBP2-52476] - Analysis using GNAS mAb against HEK293 (1) and GNAS (AA: 42-188)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-7G6G5-Immunocytochemistry-NBP2-52476-img0004.jpg)
![ELISA: G protein alpha Antibody (7G6G5) [NBP2-52476] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-7G6G5-ELISA-NBP2-52476-img0005.jpg)
![Western Blot: G protein alpha Antibody [NBP1-89754] - Analysis in human plasma.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-89754-img0009.jpg "Western Blot: G protein alpha Antibody [NBP1-89754] - Analysis in human plasma.")
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-89754] - Staining of human skin shows no positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89754-img0008.jpg)
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-89754] - Staining of human duodenum shows moderate positivity in mucus in glandular cells.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89754-img0005.jpg)
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-89754] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89754-img0006.jpg)
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-89754] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89754-img0007.jpg)
![Western Blot: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Western blot analysis of G protein alpha on different lysates with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 ug/Lane) Lane 2: RAW264.7 cell lysate (20 ug/Lane) Lane 3: C6 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 44 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32396) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32396_mouse-g-protein-alpha-mab-a8f11-r-2782024163168.jpg "Western Blot: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Western blot analysis of G protein alpha on different lysates with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 ug/Lane) Lane 2: RAW264.7 cell lysate (20 ug/Lane) Lane 3: C6 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 44 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32396) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32396) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32396_mouse-g-protein-alpha-mab-a8f11-r-298202414161854.jpg)
![Immunocytochemistry/ Immunofluorescence: G protein alpha Antibody (A8F11-R) [NBP3-32396] - Immunocytochemistry analysis of MCF7 cells labeling G protein alpha with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-G protein alpha antibody (NBP3-32396) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32396_mouse-g-protein-alpha-mab-a8f11-r-29202410234228.jpg)
![Western Blot: G protein alpha Antibody [NBP1-89753] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-225](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-NBP1-89753-img0005.jpg "Western Blot: G protein alpha Antibody [NBP1-89753] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-225")
![Immunohistochemistry-Paraffin: G protein alpha Antibody [NBP1-89753] - Staining of human colon shows distinct positivity in microvilli of glandular cells.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89753-img0001.jpg)
![Western Blot: G protein alpha Antibody - R201H Mutant [NBP3-13455] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha (R201H Mutant) antibody (NBP3-13455) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody---R201H-Mutant-Western-Blot-NBP3-13455-img0001.jpg "Western Blot: G protein alpha Antibody - R201H Mutant [NBP3-13455] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha (R201H Mutant) antibody (NBP3-13455) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: G protein alpha Antibody [H00002778-D01P] - Analysis of GNAS expression in transfected 293T cell line by GNAS polyclonal antibody.Lane 1: GNAS transfected lysate(45.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Western-Blot-H00002778-D01P-img0003.jpg "Western Blot: G protein alpha Antibody [H00002778-D01P] - Analysis of GNAS expression in transfected 293T cell line by GNAS polyclonal antibody.Lane 1: GNAS transfected lysate(45.70 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: G protein alpha Antibody [H00002778-D01P] - Analysis of protein-protein interactions between GNAS and ADCY2. HeLa cells were stained with anti-GNAS rabbit purified polyclonal 1:1200 and anti-ADCY2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Proximity-Ligation-Assay-H00002778-D01P-img0002.jpg)
![SDS-Page: Recombinant Human G protein alpha Protein [H00002778-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002778-P01-1.jpg "SDS-Page: Recombinant Human G protein alpha Protein [H00002778-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry: G protein alpha Antibody [NBP1-89752] - Staining of human fallopian tube shows distinct positivity in cilia.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody-Immunohistochemistry-NBP1-89752-img0003.jpg "Immunohistochemistry: G protein alpha Antibody [NBP1-89752] - Staining of human fallopian tube shows distinct positivity in cilia.")
![Western Blot: G protein alpha Antibody - R201C Mutant [NBP3-13454] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha (R201C Mutant) antibody (NBP3-13454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/G-protein-alpha-Antibody---R201C-Mutant-Western-Blot-NBP3-13454-img0001.jpg "Western Blot: G protein alpha Antibody - R201C Mutant [NBP3-13454] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha (R201C Mutant) antibody (NBP3-13454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")