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![Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Analysis in human prostate and skeletal muscle tissues. Corresponding NDRG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-86636-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Analysis in human prostate and skeletal muscle tissues. Corresponding NDRG1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in human cell lines HEK293 and MCF-7. Corresponding NDRG1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Western-Blot-NBP1-86636-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody [NBP1-86636] - Staining of human cell line U-2 OS shows localization to cytosol & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86636-img0008.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-86636-img0016.jpg)
![Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Western-Blot-NBP1-86636-img0007.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human colon shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-86636-img0013.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-86636-img0014.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-86636-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (2D7) [H00010397-M03] - Analysis of monoclonal antibody to NDRG1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NDRG1-Antibody-2D7-Immunocytochemistry-Immunofluorescence-H00010397-M03-img0004.jpg "Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (2D7) [H00010397-M03] - Analysis of monoclonal antibody to NDRG1 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (2D7) [H00010397-M03] - Analysis of monoclonal antibody to NDRG1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 0.7 ug/ml](http://images.novusbio.com/fullsize/NDRG1-Antibody-2D7-Immunohistochemistry-Paraffin-H00010397-M03-img0005.jpg)
![ELISA: NDRG1 Antibody (2D7) [H00010397-M03] - Detection limit for recombinant GST tagged NDRG1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDRG1-Antibody-2D7-ELISA-H00010397-M03-img0003.jpg)
![Western Blot: NDRG1 Antibody [NB300-839] - (0.1ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Western-Blot-NB300-839-img0005.jpg "Western Blot: NDRG1 Antibody [NB300-839] - (0.1ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NB300-839-img0007.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - (0.5ug/ml) staining of paraffin embedded Human hepatocelluar carcinoma. Data obtained from C. Furer, Visceral Surgery, University of Bern, Switzerland.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NB300-839-img0004.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Immunohistochemistry-Paraffin-NB300-839-img0006.jpg)
![Western Blot: NDRG1 Antibody (2C6K6) [NBP3-16321] - Western blot analysis of extracts of various cell lines, using NDRG1 Rabbit mAb (NBP3-16321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NDRG1-Antibody-2C6K6-Western-Blot-NBP3-16321-img0004.jpg "Western Blot: NDRG1 Antibody (2C6K6) [NBP3-16321] - Western blot analysis of extracts of various cell lines, using NDRG1 Rabbit mAb (NBP3-16321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry of paraffin-embedded mouse kidney using NDRG1 Rabbit mAb (NBP3-16321) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDRG1-Antibody-2C6K6-Immunohistochemistry-Paraffin-NBP3-16321-img0003.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry of paraffin-embedded rat kidney using NDRG1 Rabbit mAb (NBP3-16321) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDRG1-Antibody-2C6K6-Immunohistochemistry-Paraffin-NBP3-16321-img0001.jpg)
![Immunohistochemistry-Paraffin: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry of paraffin-embedded human esophageal cancer using NDRG1 Rabbit mAb (NBP3-16321) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NDRG1-Antibody-2C6K6-Immunohistochemistry-Paraffin-NBP3-16321-img0002.jpg)
![Immunohistochemistry: NDRG1 Antibody (4C8) [NBP3-26118] - Image of NDRG1 Antibody (4C8) diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26118_rabbit-ndrg1-mab-4c8-26220241547567.jpg "Immunohistochemistry: NDRG1 Antibody (4C8) [NBP3-26118] - Image of NDRG1 Antibody (4C8) diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: NDRG1 Antibody (4C8) [NBP3-26118] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissue. All lanes: NDRG1 Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36, 34 KDa Observed band size: 43 KDa](http://images.novusbio.com/fullsize/nbp3-26118_rabbit-ndrg1-mab-4c8-2822024114094.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (4C8) [NBP3-26118] - Staining of Hela cells with NDRG1 Antibody (4C8) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26118_rabbit-ndrg1-mab-4c8-2822024131229.jpg)
![Western Blot: NDRG1 Antibody (11G4) [NBP2-59415] - Lane 1: LnCaP cell lysates, Lane 2: HeLa cell lysates, Lane 3: PC3 cell lysates, Lane 4: Hep3B cell lysates, Lane 5: HT1080 cell lysates, Lane 6: Jurkat cell lysates, Lane 7: SW480 cell lysates](http://images.novusbio.com/fullsize/NDRG1-Antibody-11G4-Western-Blot-NBP2-59415-img0001.jpg "Western Blot: NDRG1 Antibody (11G4) [NBP2-59415] - Lane 1: LnCaP cell lysates, Lane 2: HeLa cell lysates, Lane 3: PC3 cell lysates, Lane 4: Hep3B cell lysates, Lane 5: HT1080 cell lysates, Lane 6: Jurkat cell lysates, Lane 7: SW480 cell lysates")
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (11G4) [NBP2-59415] - Analysis of NDRG1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NDRG1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/NDRG1-Antibody-11G4-Immunocytochemistry-Immunofluorescence-NBP2-59415-img0002.jpg)
![Western Blot: NDRG1 Antibody [NBP3-03516] - Analysis of extracts from normal (control) and NDRG1 knockout (KO) 293T cells, using NDRG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/NDRG1-Antibody-Western-Blot-NBP3-03516-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDRG1 Antibody [NBP3-03516] - Analysis of extracts from normal (control) and NDRG1 knockout (KO) 293T cells, using NDRG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: NDRG1 Overexpression Lysate (Adult Normal) [NBL1-13529] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDRG1.](http://images.novusbio.com/fullsize/NDRG1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13529-img0002.jpg "Western Blot: NDRG1 Overexpression Lysate (Adult Normal) [NBL1-13529] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDRG1.")
![Western Blot: NDRG1 Overexpression Lysate (Adult Normal) [NBP2-09571] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDRG1.](http://images.novusbio.com/fullsize/NDRG1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09571-img0001.jpg "Western Blot: NDRG1 Overexpression Lysate (Adult Normal) [NBP2-09571] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDRG1.")
![SDS-Page: NDRG1 Protein [NBP1-30231] - NDRG1, 43.9 kDa (402aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/NDRG1-Protein-SDS-Page-NBP1-30231-img0001.jpg "SDS-Page: NDRG1 Protein [NBP1-30231] - NDRG1, 43.9 kDa (402aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")