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![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart muscle and pancreas tissues. Corresponding Myosin Light Chain 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85541-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart muscle and pancreas tissues. Corresponding Myosin Light Chain 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart tissue.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Western-Blot-NBP1-85541-img0011.jpg)
![Immunohistochemistry-Frozen: Myosin Light Chain 2 Antibody [NBP1-85541] - Mlc2v staining on E11.5 mouse Right Ventricle. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1ug/mL. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Frozen-NBP1-85541-img0014.jpg)
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85541-img0009.jpg)
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85541-img0010.jpg)
![Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541] - Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-NBP1-85541-img0012.jpg)
![Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP1-85541] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-NBP1-85541-img0013.jpg)
![Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse heart tissue lysate](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B2-Western-Blot-NBP1-30249-img0001.jpg "Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse heart tissue lysate")
![Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B2-Immunocytochemistry-Immunofluorescence-NBP1-30249-img0002.jpg)
![Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B2-Flow-Cytometry-NBP1-30249-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Immunocytochemistry-Immunofluorescence-H00004633-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of MYL2 transfected lysate using anti-MYL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYL2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Immunoprecipitation-H00004633-M01-img0006.jpg)
![Sandwich ELISA: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Detection limit for recombinant GST tagged MYL2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Sandwich-ELISA-H00004633-M01-img0007.jpg)
![Western Blot: Myosin Light Chain 2 Antibody [NBP2-99552] - Anti-Myosin Light Chain 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Lane B: Mouse muscle tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Western-Blot-NBP2-99552-img0006.jpg "Western Blot: Myosin Light Chain 2 Antibody [NBP2-99552] - Anti-Myosin Light Chain 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Lane B: Mouse muscle tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in rat skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99552-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99552-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99552-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99552-img0004.jpg)
![Western Blot: Myosin Light Chain 2 Antibody (5M6Q8) [NBP3-16693] - Western blot analysis of extracts of various cell lines, using Myosin Light Chain 2 Rabbit mAb (NBP3-16693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-5M6Q8-Western-Blot-NBP3-16693-img0003.jpg "Western Blot: Myosin Light Chain 2 Antibody (5M6Q8) [NBP3-16693] - Western blot analysis of extracts of various cell lines, using Myosin Light Chain 2 Rabbit mAb (NBP3-16693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: Myosin Light Chain 2 Antibody (5M6Q8) [NBP3-16693] - Immunofluorescence analysis of mouse heart using Myosin Light Chain 2 Rabbit mAb (NBP3-16693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-5M6Q8-Immunohistochemistry-NBP3-16693-img0002.jpg)
![Immunohistochemistry: Myosin Light Chain 2 Antibody (5M6Q8) [NBP3-16693] - Immunofluorescence analysis of rat heart using Myosin Light Chain 2 Rabbit mAb (NBP3-16693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-5M6Q8-Immunohistochemistry-NBP3-16693-img0001.jpg)
![Western Blot: Myosin Light Chain 2 Antibody (7C9) [NBP1-28871] - Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-7C9-Western-Blot-NBP1-28871-img0002.jpg "Western Blot: Myosin Light Chain 2 Antibody (7C9) [NBP1-28871] - Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.")
![Western Blot: Myosin Light Chain 2 Antibody (RM484)[NBP3-18830]Western Blot of mouse heart tissue lysate, using Anti-Phospho-Myl2 (Ser19) rabbit monoclonal antibody (clone RM484) at a 1:1000 dilution. The phospho-specificity of RM484 was verified by peptide blocking using a control non-phosphopeptide or phosphopeptide targeting residue Ser19.](http://images.novusbio.com/fullsize/nbp3-18830_rabbit-myosin-light-chain-2-mab-rm484-16112023130185.png "Western Blot: Myosin Light Chain 2 Antibody (RM484)[NBP3-18830]Western Blot of mouse heart tissue lysate, using Anti-Phospho-Myl2 (Ser19) rabbit monoclonal antibody (clone RM484) at a 1:1000 dilution. The phospho-specificity of RM484 was verified by peptide blocking using a control non-phosphopeptide or phosphopeptide targeting residue Ser19.")
![Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP2-13634] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-Immunohistochemistry-NBP2-13634-img0002.jpg "Immunohistochemistry: Myosin Light Chain 2 Antibody [NBP2-13634] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")
![SDS-Page: Recombinant Human Myosin Light Chain 2 Protein [NBC1-18536] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 20.9 kDa (186aa), confirmed by MALDI-TOF.Concentration: 0.5 mg/ml (determined by Bradford assay).](http://images.novusbio.com/fullsize/Recombinant-Human-Myosin-Light-Chain-2-Protein-SDS-Page-NBC1-18536-img0001.jpg "SDS-Page: Recombinant Human Myosin Light Chain 2 Protein [NBC1-18536] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 20.9 kDa (186aa), confirmed by MALDI-TOF.Concentration: 0.5 mg/ml (determined by Bradford assay).")
![Western Blot: Myosin Light Chain 2 Overexpression Lysate (Adult Normal) [NBL1-13424] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Light Chain 2.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13424-img0002.jpg "Western Blot: Myosin Light Chain 2 Overexpression Lysate (Adult Normal) [NBL1-13424] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Light Chain 2.")