| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | FAB2319A | |||
| Biotin | BAF2319 | |||
| Fluorescein | FAB2319F | |||
| PerCP (Peridinin-chlorophyll Protein Complex) | FAB2319C | |||
| Phycoerythrin | FAB2319P | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB2319C | |||
| Carrier Free | FAB2319F | |||
| Carrier Free | FAB2319P | |||
| Carrier Free | FAB2319A | |||
| Carrier Free | AF2319-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3328-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97662-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-23442-25ug | |||
| BSA Free | NBP3-23442-100ug | |||
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0001.jpg "Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 ug/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown).")
![Western Blot: LAG-3 Antibody Pack [NBP3-11743] - Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated or treated (+) with 1 ug/mL PHA for 5 days and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LAG-3 at approximately 60-75 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Western-Blot-NBP3-11743-img0002.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 ug/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0003.jpg)
![Flow Cytometry: LAG-3 Antibody Pack [NBP3-11743] - Analysis using the FITC conjugate of NBP1-97657. Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4).](http://images.novusbio.com/fullsize/LAG-3-Antibody-Pack-Flow-Cytometry-NBP3-11743-img0005.jpg)
![Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-11E3-Flow-Cytometry-NBP1-97662-img0001.jpg "Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.")
![Western Blot: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Western-Blot-NBP2-76403-img0003.jpg "Western Blot: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate (observed molecular weight of ~ 57 KDa). Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) and rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunoprecipitation-NBP2-76403-img0004.jpg)
![Immunocytochemistry: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunocytochemistry-NBP2-76403-img0002.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR028F) [NBP2-76403] - Section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR028F-Immunohistochemistry-NBP2-76403-img0001.jpg)
![Western Blot: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Western-Blot-NBP2-76402-img0007.jpg "Western Blot: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate (observed molecular weight of ~ 57 KDa). Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate (observed molecular weight of ~ 57 KDa). Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402) and another rabbit anti-LAG3 recombinant monoclonal.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunoprecipitation-NBP2-76402-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunocytochemistry-NBP2-76402-img0002.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunocytochemistry-Immunofluorescence-NBP2-76402-img0010.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE tonsil by IHC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402), Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.](http://images.novusbio.com/fullsize/LAG-3-Antibody-BLR027F-Immunohistochemistry-NBP2-76402-img0001.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining in human tonsil and cerebral cortex tissues using anti-LAG3 antibody. Corresponding LAG3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining in human tonsil and cerebral cortex tissues using anti-LAG3 antibody. Corresponding LAG3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0006.jpg)
![Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/LAG-3-Antibody-Immunohistochemistry-Paraffin-NBP1-85781-img0005.jpg)
![Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Western-Blot-NBP3-05777-img0001.jpg "Western Blot: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Western blot analysis of LAG-3 mAb (L4-PL33): Lane 1: MW marker, Lane 2: LAG-3 (human): Fc (human), (recombinant).")
![Immunohistochemistry-Paraffin: LAG-3 Antibody (L4-PL33) - Azide Free [NBP3-05777] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil tissue stained with LAG-3 (human) recombinant mAb (L4-PL33) at 6ug/ml.](http://images.novusbio.com/fullsize/LAG-3-Antibody-L4-PL33-Azide-Free-Immunohistochemistry-Paraffin-NBP3-05777-img0002.jpg)
