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![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0006.jpg "Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunofluorescence analysis of NIH-3T3 cells using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Immunocytochemistry-Immunofluorescence-NBP3-16632-img0004.jpg)
![Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded mouse brain using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Immunohistochemistry-Paraffin-NBP3-16632-img0003.jpg)
![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of various cell lines, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0005.jpg)
![Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Rat brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Western-Blot-NBP3-16632-img0007.jpg)
![Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded rat spleen using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Immunohistochemistry-Paraffin-NBP3-16632-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded human appendix using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CHD3-Antibody-8Z6L6-Immunohistochemistry-Paraffin-NBP3-16632-img0002.jpg)
![Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/CHD3-Antibody-Western-Blot-NB100-60412-img0005.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunohistochemistry-Paraffin-NB100-60412-img0007.jpg)
![Western Blot: CHD3 Antibody [NBP3-13040] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody (NBP3-13040) diluted at 1:1000.](http://images.novusbio.com/fullsize/CHD3-Antibody-Western-Blot-NBP3-13040-img0001.jpg "Western Blot: CHD3 Antibody [NBP3-13040] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody (NBP3-13040) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CHD3 Antibody [NBP3-13040] - CHD3 antibody detects CHD3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CHD3 protein stained by CHD3 antibody (NBP3-13040) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13040-img0002.jpg)
![Immunohistochemistry-Paraffin: CHD3 Antibody [NBP3-13040] - CHD3 antibody detects CHD3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHD3 antibody (NBP3-13040) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunohistochemistry-Paraffin-NBP3-13040-img0003.jpg)
![Western Blot: CHD3 Antibody (SR1409) [NBP3-21768] - Western blot analysis using NBP3-21768 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21768_rabbit-chd3-mab-sr1409-57202316112189.jpg "Western Blot: CHD3 Antibody (SR1409) [NBP3-21768] - Western blot analysis using NBP3-21768 in K562 cell lysate.")
![Western Blot: CHD3 Antibody (SR1410) [NBP3-22441] - Western blot analysis using NBP3-22441 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22441_rabbit-chd3-mab-sr1410-6720239533790.jpg "Western Blot: CHD3 Antibody (SR1410) [NBP3-22441] - Western blot analysis using NBP3-22441 in HeLa cell lysate.")
![Western Blot: CHD3 Antibody [NBP3-37987] - Western blot analysis of various lysates using CHD3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37987_rabbit-chd3-pab-9122024159595.jpg "Western Blot: CHD3 Antibody [NBP3-37987] - Western blot analysis of various lysates using CHD3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.")
![SDS-Page: Recombinant Human CHD3 Protein [H00001107-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001107-Q01-1.jpg "SDS-Page: Recombinant Human CHD3 Protein [H00001107-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: CHD3 Antibody (5E12) [H00001107-M01] - Analysis of monoclonal antibody to CHD3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CHD3-Antibody-5E12-Immunohistochemistry-Paraffin-H00001107-M01-img0003.jpg "Immunohistochemistry-Paraffin: CHD3 Antibody (5E12) [H00001107-M01] - Analysis of monoclonal antibody to CHD3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.")
![Immunohistochemistry-Paraffin: CHD3 Antibody [NBP2-14479] - Staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunohistochemistry-Paraffin-NBP2-14479-img0001.jpg "Immunohistochemistry-Paraffin: CHD3 Antibody [NBP2-14479] - Staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.")
![Immunocytochemistry/Immunofluorescence: CHD3 Antibody [NBP2-57564] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57564-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHD3 Antibody [NBP2-57564] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")
![ELISA: Mouse CHD3 ELISA Kit (Colorimetric) [NBP2-75267] - Samples were spiked with high concentrations of Mouse CHD3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75267_mouse-chd3-elisa-kit-colorimetric-132202416184891.jpg "ELISA: Mouse CHD3 ELISA Kit (Colorimetric) [NBP2-75267] - Samples were spiked with high concentrations of Mouse CHD3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CHD3 ELISA Kit (Colorimetric) [NBP2-75267] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75267_mouse-chd3-elisa-kit-colorimetric-432024855094.jpg)
![ELISA: Rat CHD3 ELISA Kit (Colorimetric) [NBP2-75268] - Samples were spiked with high concentrations of Rat CHD3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75268_rat-chd3-elisa-kit-colorimetric-132202416254049.jpg "ELISA: Rat CHD3 ELISA Kit (Colorimetric) [NBP2-75268] - Samples were spiked with high concentrations of Rat CHD3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CHD3 ELISA Kit (Colorimetric) [NBP2-75268] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75268_rat-chd3-elisa-kit-colorimetric-432024855033.jpg)