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![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0005.jpg "Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (NBP2-59376). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (NBP2-59376) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)
![SDS-Page: Recombinant Human ATP7A Protein [H00000538-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATP7A-Protein-SDS-Page-H00000538-Q01-img0001.jpg "SDS-Page: Recombinant Human ATP7A Protein [H00000538-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: ATP7A Antibody [NBP1-82631] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/ATP7A-Antibody-Immunohistochemistry-Paraffin-NBP1-82631-img0004.jpg "Immunohistochemistry-Paraffin: ATP7A Antibody [NBP1-82631] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and glial cells.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody [NBP2-56307] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATP7A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56307-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP7A Antibody [NBP2-56307] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Western Blot: ATP7A Antibody [NBP3-10309] - Western blot analysis using NBP3-10309 on Human HT1080 as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/ATP7A-Antibody-Western-Blot-NBP3-10309-img0001.jpg "Western Blot: ATP7A Antibody [NBP3-10309] - Western blot analysis using NBP3-10309 on Human HT1080 as a positive control. Antibody Titration: 0.2-1 ug/ml")
![Western Blot: ATP7A Antibody [NBP3-10578] - Western blot analysis of ATP7A in Fetal Liver lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/ATP7A-Antibody-Western-Blot-NBP3-10578-img0001.jpg "Western Blot: ATP7A Antibody [NBP3-10578] - Western blot analysis of ATP7A in Fetal Liver lysates. Antibody dilution at 1.0ug/ml")