| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37520-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37518-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70217 | |||
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - ASS1 was successfully silenced in H4-II-E-C3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0013.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - ASS1 was successfully silenced in H4-II-E-C3 cells. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88868-img0007.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human skin shows moderate cytoplasmic positivity in a subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0023.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - Analysis in human cell lines MCF-7 and U-251MG using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0012.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0008.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney, liver, lymph node and urinary bladder using Anti-ASS1 antibody NBP1-88868 (A) shows similar protein distribution across tissues to independent antibody NBP1-88867 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0014.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0015.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney using Anti-ASS1 antibody NBP1-88868.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0017.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node using Anti-ASS1 antibody NBP1-88868.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0018.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Analysis in human kidney and lymph node tissues using NBP1-88868 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0019.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney, liver, lymph node and skin using Anti-ASS1 antibody NBP1-88868 (A) shows similar protein distribution across tissues to independent antibody NBP1-88867 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0020.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0021.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0022.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0024.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0005.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00153-img0006.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0003.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Flow-Cytometry-NBP1-00153-img0007.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0004.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0002.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney, liver, lymph node and urinary bladder using Anti-ASS1 antibody NBP1-88867 (A) shows similar protein distribution across tissues to independent antibody NBP1-88868 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Argininosuccinate Synthase Antibody [NBP1-88867] - Analysis in human cell lines MCF-7 and U-251MG using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88867-img0011.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human skin shows strong cytoplasmic positivity in a subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0023.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node using Anti-ASS1 antibody NBP1-88867.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0017.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0019.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0021.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0022.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0024.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Western-Blot-NBP2-37520-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Hela cells using ASS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Immunocytochemistry-Immunofluorescence-NBP2-37520-img0004.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Jurkat cells using ASS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Flow-Cytometry-NBP2-37520-img0006.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Western-Blot-NBP2-37520-img0002.jpg)
![ELISA: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-ELISA-NBP2-37520-img0001.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Western-Blot-NBP2-37518-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.")
![Immunohistochemistry: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Immunohistochemistry-NBP2-37518-img0005.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Western-Blot-NBP2-37518-img0002.jpg)
![Immunohistochemistry: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ASS1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Immunohistochemistry-NBP2-37518-img0004.jpg)
![ELISA: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-ELISA-NBP2-37518-img0001.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Western blot detection of Argininosuccinate Synthase in Hela cell lysates using NBP3-19945 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Western-Blot-NBP3-19945-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Western blot detection of Argininosuccinate Synthase in Hela cell lysates using NBP3-19945 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Immunofluorescence of Argininosuccinate Synthase (green) in Hela cells using NBP3-19945 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Immunocytochemistry-Immunofluorescence-NBP3-19945-img0001.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Immunohistochemistry of Argininosuccinate Synthase in paraffin-embedded Human breast cancer tissue using NBP3-19945 at dilution 1/1](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Immunohistochemistry-Paraffin-NBP3-19945-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Western blot analysis of Argininosuccinate Synthase on different lysates with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: SiHa cell lysate (10 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32051) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-278202416331278.jpg "Western Blot: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Western blot analysis of Argininosuccinate Synthase on different lysates with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: SiHa cell lysate (10 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32051) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-2982024142090.jpg)
![Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Immunocytochemistry analysis of MCF-7 cells labeling Argininosuccinate Synthase with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-2920241033404.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Western blot analysis of extracts of various cell lines, using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-4W1M8-Western-Blot-NBP3-16749-img0002.jpg "Western Blot: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Western blot analysis of extracts of various cell lines, using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Immunofluorescence analysis of NIH-3T3 cells using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-4W1M8-Immunocytochemistry-Immunofluorescence-NBP3-16749-img0001.jpg)
![Immunohistochemistry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Image of Argininosuccinate Synthase Antibody (18G11) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.56% DAB.](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-262202415475626.jpg "Immunohistochemistry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Image of Argininosuccinate Synthase Antibody (18G11) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.56% DAB.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Staining of Hela with Argininosuccinate Synthase Antibody (18G11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 516-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-28220241247517.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Overlay Peak curve showing Jurkat cells stained with Argininosuccinate Synthase Antibody (18G11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-282202414312780.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-31522] - A. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE Argininosuccinate Synthase antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-31522-img0004.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-31522] - A. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE Argininosuccinate Synthase antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-31522] - Cal27 xenograft, using ASS1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-31522-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis in MCF-7 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1G11-Western-Blot-NBP2-59443-img0001.jpg "Western Blot: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis in MCF-7 cell lysates (40ug), resolved by SDS-PAGE.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis of ASS1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ASS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1G11-Immunocytochemistry-Immunofluorescence-NBP2-59443-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (1B10) [NBP2-46551] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Synthase.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1B10-Western-Blot-NBP2-46551-img0001.jpg "Western Blot: Argininosuccinate Synthase Antibody (1B10) [NBP2-46551] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Synthase.")