Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Knockdown Validated: TSPAN1 Antibody [NBP2-33867] - Knockdown tetraspanin 1 (TSPAN1) promoted molecular characterization of epithelial-to-mesenchymal transition state in alveolar epithelial cells. Interference efficiency of siRNA for TSPAN1 was identified in 293T cells by Western blotting. Four siRNA sequences, named si-TSPAN1-1, si-TSPAN1-2, si-TSPAN1-3 and si-TSPAN1-4, were designed to aim four target sites for TSPAN1. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.14258), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Knockdown-Validated-NBP2-33867-img0018.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: TSPAN1 Antibody [NBP2-33867] - Knockdown tetraspanin 1 (TSPAN1) promoted molecular characterization of epithelial-to-mesenchymal transition state in alveolar epithelial cells. Interference efficiency of siRNA for TSPAN1 was identified in 293T cells by Western blotting. Four siRNA sequences, named si-TSPAN1-1, si-TSPAN1-2, si-TSPAN1-3 and si-TSPAN1-4, were designed to aim four target sites for TSPAN1. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.14258), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TSPAN1 Antibody [NBP2-33867] - Analysis in human cell line PC-3 and human cell line HeLa.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Western-Blot-NBP2-33867-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP2-33867] - Analysis in human kidney and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33867-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TSPAN1 Antibody [NBP2-33867] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33867-img0010.jpg)
![Western Blot: TSPAN1 Antibody [NBP2-33867] - Screening of gene in idiopathic pulmonary fibrosis (IPF) and identification of tetraspanin 1 (TSPAN1) in vivo and vitro. Western blotting was used to detect the protein expression of TSPAN1 in A549, alveolar epithelial type II (ATII) cells, MRC-5 and rat fibroblasts. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.14258), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Western-Blot-NBP2-33867-img0019.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP2-33867] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33867-img0016.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP2-33867] - Staining of human colon shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33867-img0013.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP2-33867] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33867-img0014.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP2-33867] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33867-img0015.jpg)
![Western Blot: TSPAN1 Antibody [NBP2-33867] - Knockdown tetraspanin 1 (TSPAN1) promoted cell migration & transforming growth factor‐ beta 1 (TGF‐ beta 1)‐induced epithelial‐to‐mesenchymal transition‐related proteins in alveolar epithelial cells. A, Wound healing assay following silencing of TSPAN1 in A549 cells. B, Quantification results of wound healing assay. Bar chart represents the ratio of every group to negative control (NC) groups. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05 vs NC). C, Transwell migration analysis by silencing TSPAN1 in A549 cells with or without TGF‐ beta 1 treatment (5 ng/mL, 16 h). D, Quantification of transwell migration analysis. The bar chart represents the numbers of migrating cells. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (**P < 0.01 vs NC). (E,G) Western blot analysis of various proteins by silencing TSPAN1 for 24 h in A549 & alveolar epithelial type II cells with or without TGF‐ beta 1 treatment. F, Quantification of the results in panel (E), & (H) quantification of the results in panel (G). The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs NC 0 h; #P < 0.05, ##P < 0.01, ###P < 0.001 vs NC 12 h; delta P < 0.05, delta delta P < 0.01, delta delta delta P < 0.001 vs NC 24 h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30869194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33867_rabbit-polyclonal-tspan1-antibody-3102024161366.jpg)
![Western Blot: TSPAN1 Antibody [NBP2-33867] - Tetraspanin 1 (TSPAN1) modulates beta‐catenin & phosphorylation of Smad2/3. Immunofluorescence staining analysis of TSPAN1 & phosphorylation of Smad2/3 after transfected with siRNA for TSPAN1 or negative control (NC) in A549 cells with or without TGF‐ beta 1 treatment (5 ng/mL transforming growth factor‐ beta 1 [TGF‐ beta 1] or control) (green: TSPAN1; red: P‐smad2/3; blue: 4′,6‐diamidino‐2‐phenylindole). Magnification: 1200X. (B‐I) Western blot analysis of various proteins after transfected with siRNA or exogenous expression plasmid for TSPAN1 or NC in A549 & alveolar epithelial type II (ATII) cells with or without TGF‐ beta 1 treatment (5 ng/mL; 0, 12, 24 h). B, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 were detected by silencing TSPAN1 in A549 cells. C, Quantification of the results in panel (A). D, The proteins expression of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 were detected, using western blotting after silencing TSPAN1 in ATII cells. E, Quantification of the results in panel (D). F, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 were detected after overexpression of TSPAN1 in A549 cells. G, Quantification of the results in panel (F). H, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 were detected by Western blotting after overexpression of TSPAN1 in ATII cells. I, Quantification of the results in panel (H). J, Phosphorylation of Smad2/3 was detected by Western blotting in intranuclear or cytoplasm after silencing TSPAN1 in A549 cells. K, Quantification of the results in panel (J). The data are expressed as the means ± SEM (n = 3), with results representative of 3 independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs NC 0 h; #P < 0.05, ##P < 0.01, ###P < 0.001 vs NC 12 h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30869194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33867_rabbit-polyclonal-tspan1-antibody-31020241611473.jpg)
![Immunocytochemistry/ Immunofluorescence: TSPAN1 Antibody [NBP2-33867] - Tetraspanin 1 (TSPAN1) modulates beta‐catenin & phosphorylation of Smad2/3. Immunofluorescence staining analysis of TSPAN1 & phosphorylation of Smad2/3 after transfected w/ siRNA for TSPAN1 or negative control (NC) in A549 cells w/ or w/out TGF‐ beta 1 treatment (5 ng/mL transforming growth factor‐ beta 1 [TGF‐ beta 1] or control) (green: TSPAN1; red: P‐smad2/3; blue: 4′,6‐diamidino‐2‐phenylindole). Magnification: 1200X. (B‐I) Western blot analysis of various proteins after transfected w/ siRNA or exogenous expression plasmid for TSPAN1 or NC in A549 & alveolar epithelial type II (ATII) cells w/ or w/out TGF‐ beta 1 treatment (5 ng/mL; 0, 12, 24 h). B, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 detected by silencing TSPAN1 in A549 cells. C, Quantification of Results in panel (A). D, The proteins expression of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 detected, using western blotting after silencing TSPAN1 in ATII cells. E, Quantification of Results in panel (D). F, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 detected after overexpression of TSPAN1 in A549 cells. G, Quantification of Results in panel (F). H, Proteins of Smad2/3, beta‐catenin & phosphorylation of Smad2/3 detected by Western blotting after overexpression of TSPAN1 in ATII cells. I, Quantification of Results in panel (H). J, Phosphorylation of Smad2/3 detected by Western blotting in intranuclear or cytoplasm after silencing TSPAN1 in A549 cells. K, Quantification of Results in panel (J). The data are expressed as the means ± SEM (n = 3), w/ results representative of 3 independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs NC 0 h; #P < 0.05, ##P < 0.01, ###P < 0.001 vs NC 12 h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30869194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33867_rabbit-polyclonal-tspan1-antibody-310202416121965.jpg)
![Western Blot: TSPAN1 Antibody [NBP2-33867] - Knockdown tetraspanin 1 (TSPAN1) promoted cell migration & transforming growth factor‐ beta 1 (TGF‐ beta 1)‐induced epithelial‐to‐mesenchymal transition‐related proteins in alveolar epithelial cells. A, Wound healing assay following silencing of TSPAN1 in A549 cells. B, Quantification results of wound healing assay. Bar chart represents the ratio of every group to negative control (NC) groups. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05 vs NC). C, Transwell migration analysis by silencing TSPAN1 in A549 cells with or without TGF‐ beta 1 treatment (5 ng/mL, 16 h). D, Quantification of transwell migration analysis. The bar chart represents the numbers of migrating cells. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (**P < 0.01 vs NC). (E,G) Western blot analysis of various proteins by silencing TSPAN1 for 24 h in A549 & alveolar epithelial type II cells with or without TGF‐ beta 1 treatment. F, Quantification of the results in panel (E), & (H) quantification of the results in panel (G). The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs NC 0 h; #P < 0.05, ##P < 0.01, ###P < 0.001 vs NC 12 h; delta P < 0.05, delta delta P < 0.01, delta delta delta P < 0.001 vs NC 24 h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30869194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33867_rabbit-polyclonal-tspan1-antibody-31020241611476.jpg)
![Western Blot: TSPAN1 Antibody [NBP1-30931] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Tetraspan 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Western-Blot-NBP1-30931-img0007.jpg "Western Blot: TSPAN1 Antibody [NBP1-30931] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Tetraspan 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TSPAN1 Antibody [NBP1-30931] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Immunohistochemistry-Paraffin-NBP1-30931-img0004.jpg)
![Western Blot: TSPAN1 Antibody [NBP1-30931] - Sample(30 ug whole cell lysate)A: H129912% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Western-Blot-NBP1-30931-img0005.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody (010) [NBP2-90167] - Immunochemical staining of human TSPAN1 in human prostate with rabbit monoclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/TSPAN1-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90167-img0002.jpg "Immunohistochemistry-Paraffin: TSPAN1 Antibody (010) [NBP2-90167] - Immunochemical staining of human TSPAN1 in human prostate with rabbit monoclonal antibody (1:10000, formalin-fixed paraffin embedded sections).")
![Flow Cytometry: TSPAN1 Antibody (010) [NBP2-90167] - Flow cytometric analysis of Human TSPAN1 expression on SW480 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human TSPAN1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-010-Flow-Cytometry-NBP2-90167-img0003.jpg)
![Immunohistochemistry-Paraffin: TSPAN1 Antibody (010) [NBP2-90167] - Immunochemical staining of human TSPAN1 in human rectal cancer with rabbit monoclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/TSPAN1-Antibody-010-Immunohistochemistry-Paraffin-NBP2-90167-img0001.jpg)
![Sandwich ELISA: TSPAN1 Antibody (3B4) [H00010103-M05] - Detection limit for recombinant GST tagged TSPAN1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-3B4-Sandwich-ELISA-H00010103-M05-img0002.jpg "Sandwich ELISA: TSPAN1 Antibody (3B4) [H00010103-M05] - Detection limit for recombinant GST tagged TSPAN1 is approximately 3ng/ml as a capture antibody.")
![Immunocytochemistry/Immunofluorescence: TSPAN1 Antibody (4/12) - Chimeric [NBP3-12061] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 4/12 (NBP3-12061) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12061, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-4-12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12061-img0001.jpg "Immunocytochemistry/Immunofluorescence: TSPAN1 Antibody (4/12) - Chimeric [NBP3-12061] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 4/12 (NBP3-12061) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12061, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![SDS-Page: Recombinant Human TSPAN1 Protein [H00010103-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010103-Q01-1.jpg "SDS-Page: Recombinant Human TSPAN1 Protein [H00010103-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TSPAN1 Antibody [NBP1-54405] - Titration: 0.2-1 ug/ml, Positive Control: MCF7 cell lysate.](http://images.novusbio.com/fullsize/TSPAN1-Antibody-Western-Blot-NBP1-54405-img0004.jpg "Western Blot: TSPAN1 Antibody [NBP1-54405] - Titration: 0.2-1 ug/ml, Positive Control: MCF7 cell lysate.")
![Western Blot: Tetraspanin-1 Overexpression Lysate (Adult Normal) [NBP2-10447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tetraspanin-1.](http://images.novusbio.com/fullsize/Tetraspanin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10447-img0001.jpg "Western Blot: Tetraspanin-1 Overexpression Lysate (Adult Normal) [NBP2-10447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tetraspanin-1.")
![ELISA: Human TSPAN1 ELISA Kit (Colorimetric) [NBP3-40212] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40212_human-tspan1-elisa-kit-colorimetric-2110202415302953.png "ELISA: Human TSPAN1 ELISA Kit (Colorimetric) [NBP3-40212] - Standard Curve Reference")
![ELISA: Human TSPAN1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40211] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40211_human-tspan1-ready-to-use-elisa-kit-colorimetric-2110202415305281.png "ELISA: Human TSPAN1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40211] - Standard Curve Reference")