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![Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TRIM2-Antibody-NB100-1218-img0005.jpg "Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-Paraffin-NB100-1218-img0003.jpg)
![Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Flow-Cytometry-NB100-1218-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TRIM2 Antibody [NBP1-81504] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81504-img0010.jpg "Immunocytochemistry/Immunofluorescence: TRIM2 Antibody [NBP1-81504] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504] - Staining in human cerebral cortex and lymph node tissues using anti-TRIM2 antibody. Corresponding TRIM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-81504-img0009.jpg)
![Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-81504-img0007.jpg)
![Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-81504-img0008.jpg)
![Western Blot: TRIM2 Antibody (2B10) [NBP2-02928] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.](http://images.novusbio.com/fullsize/TRIM2-Antibody-2B10-Western-Blot-NBP2-02928-img0009.jpg "Western Blot: TRIM2 Antibody (2B10) [NBP2-02928] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.")
![Flow Cytometry: TRIM2 Antibody (2B10) [NBP2-02928] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TRIM2-Antibody-2B10-Flow-Cytometry-NBP2-02928-img0008.jpg)
![Western Blot: TRIM2 Antibody (2B10) [NBP2-02928] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.](http://images.novusbio.com/fullsize/TRIM2-Antibody-2B10-Western-Blot-NBP2-02928-img0005.jpg)
![Flow Cytometry: TRIM2 Antibody (2B10) [NBP2-02928] - Analysis of Hela cells, using anti-TRIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRIM2-Antibody-2B10-Flow-Cytometry-NBP2-02928-img0006.jpg)
![Flow Cytometry: TRIM2 Antibody (2B10) [NBP2-02928] - Analysis of Jurkat cells, using anti-TRIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRIM2-Antibody-2B10-Flow-Cytometry-NBP2-02928-img0007.jpg)
![Immunohistochemistry: TRIM2 Antibody [NBP1-81505] - Staining of human cerebellum shows cytoplasmic positivity Purkinje cells.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-NBP1-81505-img0005.jpg "Immunohistochemistry: TRIM2 Antibody [NBP1-81505] - Staining of human cerebellum shows cytoplasmic positivity Purkinje cells.")
![Western Blot: TRIM2 Antibody [NBP2-20709] - Analysis of TRIM2 expression in transfected 293T cell line (20ug of whole cell ltsate) A: Non-transfected lysate. B: TRIM2 transfected lysate. 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TRIM2-Antibody-Western-Blot-NBP2-20709-img0001.jpg "Western Blot: TRIM2 Antibody [NBP2-20709] - Analysis of TRIM2 expression in transfected 293T cell line (20ug of whole cell ltsate) A: Non-transfected lysate. B: TRIM2 transfected lysate. 7.5% SDS PAGE diluted at 1:1000")
![Western Blot: TRIM2 Overexpression Lysate (Adult Normal) [NBL1-17280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRIM2.](http://images.novusbio.com/fullsize/TRIM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17280-img0002.jpg "Western Blot: TRIM2 Overexpression Lysate (Adult Normal) [NBL1-17280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRIM2.")
![Western Blot: TRIM2 Overexpression Lysate (Adult Normal) [NBP2-09504] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRIM2.](http://images.novusbio.com/fullsize/TRIM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09504-img0001.jpg "Western Blot: TRIM2 Overexpression Lysate (Adult Normal) [NBP2-09504] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRIM2.")