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![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Topoisomerase I Antibody [NBP1-90365] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-90365-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody [NBP1-90365] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90365-img0014.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0019.jpg)
![Western Blot: Topoisomerase I Antibody [NBP1-90365] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-90365-img0008.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0016.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0017.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human placenta shows very strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0018.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in JAR.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0014.jpg "Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in JAR.")
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1~10 ug/ml.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Immunohistochemistry-Paraffin-H00007150-M01-img0011.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in HeLa.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0013.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in human ovarian cancer.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Immunohistochemistry-Paraffin-H00007150-M01-img0009.jpg)
![ELISA: Topoisomerase I Antibody (1A1) [H00007150-M01] - Detection limit for recombinant GST tagged TOP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-ELISA-H00007150-M01-img0016.jpg)
![Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Western blot analysis of extracts of Mouse thymus, using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Western-Blot-NBP3-15450-img0006.jpg "Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Western blot analysis of extracts of Mouse thymus, using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Human thyroid cancer using DNA topoisomerase I (Topoisomerase I) Rabbit mAb (NBP3-15450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Immunohistochemistry-Paraffin-NBP3-15450-img0001.jpg)
![Immunoprecipitation: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Topoisomerase I antibody (NBP3-15450). Western blot was performed from the immunoprecipitate using Topoisomerase I antibody (NBP3-15450) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-3D4W6-Immunoprecipitation-NBP3-15450-img0007.jpg)
![Western Blot: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Analysis of various lysates using DNA topoisomerase I (DNA topoisomerase I (TOP1)) Rabbit mAb at 1：1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15450_rabbit-topoisomerase-i-mab-3d4w6-western-blot-15102024111447..jpg)
![Immunocytochemistry/Immunofluorescence: Topoisomerase I Antibody (3D4W6) [NBP3-15450] - Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15450_rabbit-topoisomerase-i-mab-3d4w6-immunocytochemistry-immunofluorescence-1510202411164..jpg)
![Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30482-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30482-img0008.jpg)
![Immunoprecipitation: Topoisomerase I Antibody [NBP1-30482] - Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 (lot NBP1-30482-3) used for IP at 6 ug per reaction. TOP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30482-2). For blotting immunoprecipitated TOP1, NBP1-30482 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunoprecipitation-NBP1-30482-img0012.jpg)
![Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2622024132372.jpg "Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-26220241447153.jpg)
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2722024856319.jpg)
![Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate. All lanes: Topoisomerase I Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 91 kDa Observed band size: 91 kDa](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-28220241144269.jpg)
![Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Overlay histogram showing HepG2 cells stained with Topoisomerase I Antibody (6D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-282202414312734.jpg)
![Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30481-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.")
![Western Blot: Topoisomerase I Overexpression Lysate (Adult Normal) [NBL1-17194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Topoisomerase I.](http://images.novusbio.com/fullsize/Topoisomerase-I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17194-img0002.jpg "Western Blot: Topoisomerase I Overexpression Lysate (Adult Normal) [NBL1-17194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Topoisomerase I.")
![Immunohistochemistry: Topoisomerase I Antibody [NBP3-30961] - Immunohistochemical staining of normal human tonsil tissue using TOP1 antibody (Cat. No. NBP3-30961) at 10 ug/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.](http://images.novusbio.com/fullsize/nbp3-30961_rabbit-topoisomerase-i-pab-811202420592143.jpg "Immunohistochemistry: Topoisomerase I Antibody [NBP3-30961] - Immunohistochemical staining of normal human tonsil tissue using TOP1 antibody (Cat. No. NBP3-30961) at 10 ug/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.")
![ELISA: Human Topoisomerase I - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38669] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38669_human-topoisomerase-i-ready-to-use-elisa-kit-color-2110202415302945.png "ELISA: Human Topoisomerase I - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38669] - Standard Curve Reference")
![ELISA: Human Topoisomerase I ELISA Kit (Colorimetric) [NBP3-38670] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38670_human-topoisomerase-i-elisa-kit-colorimetric-2110202415384031.png "ELISA: Human Topoisomerase I ELISA Kit (Colorimetric) [NBP3-38670] - Standard Curve Reference")