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![Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PITX1-Antibody-5G4-Western-Blot-H00005307-M01-img0009.jpg "Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi ( Cat # H00005307-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 (Cat # H00005307-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PITX1-Antibody-5G4-Western-Blot-H00005307-M01-img0006.jpg)
![Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - PITX1 monoclonal antibody (M01), clone 5G4 Analysis of PITX1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PITX1-Antibody-5G4-Western-Blot-H00005307-M01-img0008.jpg)
![Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PITX1-Antibody-5G4-Western-Blot-H00005307-M01-img0010.jpg)
![Sandwich ELISA: PITX1 Antibody (5G4) [H00005307-M01] - Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PITX1-Antibody-5G4-Sandwich-ELISA-H00005307-M01-img0007.jpg)
![Western Blot: PITX1 Antibody [H00005307-D01P] - Analysis of PITX1 expression in mouse brain.](http://images.novusbio.com/fullsize/PITX1-Antibody-Western-Blot-H00005307-D01P-img0006.jpg "Western Blot: PITX1 Antibody [H00005307-D01P] - Analysis of PITX1 expression in mouse brain.")
![Immunocytochemistry/Immunofluorescence: PITX1 Antibody [H00005307-D01P] - Analysis of antibody to PITX1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PITX1-Antibody-Immunocytochemistry-Immunofluorescence-H00005307-D01P-img0004.jpg)
![Western Blot: PITX1 Antibody [H00005307-D01P] - Analysis of PITX1 expression in human placenta.](http://images.novusbio.com/fullsize/PITX1-Antibody-Western-Blot-H00005307-D01P-img0005.jpg)
![Western Blot: PITX1 Antibody [H00005307-D01P] - Analysis of PITX1 expression in transfected 293T cell line by PITX1 polyclonal antibody.Lane 1: PITX1 transfected lysate(34.10 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PITX1-Antibody-Western-Blot-H00005307-D01P-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PITX1 Antibody [NBP1-88644] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PITX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88644-img0010.jpg "Immunocytochemistry/Immunofluorescence: PITX1 Antibody [NBP1-88644] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.")
![Western Blot: PITX1 Antibody [NB100-1546] - Detection of Human PITX by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody NB100-1546 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PITX1-Antibody-Western-Blot-NB100-1546-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: PITX1 Antibody [NB100-1546] - Detection of Human PITX by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody NB100-1546 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>PITX1 Antibody [NB100-1546] - Detection of human PITX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PITX antibody NB100-1546 (lot NB100-1546-2) used for IP at 6 ug per reaction. PITX was also immunoprecipitated by a previous lot of this antibody NB100-1546-1. For blotting immunoprecipitated PITX, A300-577A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PITX1-Antibody-NB100-1546-img0004.jpg)
![Western Blot: PITX1 Antibody (6D4) [H00005307-M02] - PITX1 monoclonal antibody (M02), clone 6D4 Analysis of PITX1 expression in A-431.](http://images.novusbio.com/fullsize/PITX1-Antibody-6D4-Western-Blot-H00005307-M02-img0004.jpg "Western Blot: PITX1 Antibody (6D4) [H00005307-M02] - PITX1 monoclonal antibody (M02), clone 6D4 Analysis of PITX1 expression in A-431.")
![Sandwich ELISA: PITX1 Antibody (6D4) [H00005307-M02] - Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PITX1-Antibody-6D4-Sandwich-ELISA-H00005307-M02-img0003.jpg)
![Western Blot: PITX1 Antibody [NBP1-82383] - Rat Liver Lysate 1.0 ug/ml, gel concentration 12%](http://images.novusbio.com/fullsize/PITX1-Antibody-Western-Blot-NBP1-82383-img0001.jpg "Western Blot: PITX1 Antibody [NBP1-82383] - Rat Liver Lysate 1.0 ug/ml, gel concentration 12%")