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![Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Western-Blot-NBP2-67767-img0008.jpg "Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0002.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0003.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0004.jpg)
![Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP2-92397-img0004.jpg "Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody [NBP2-92397] - Analysis of U-2 OS cells using ATF5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92397-img0003.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Rat ovary using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody [NBP2-92397] - Analysis of C6 cells using ATF5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92397-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody [NBP2-92397] - Analysis of L929 cells using ATF5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92397-img0002.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Mouse lung using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0005.jpg)
![Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0002.jpg "Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ATF5 Antibody [NBP1-51908] - Analysis of ATF5 antibody in human heart lysate (RIPA buffer, 35 ug total loaded per lane) using NBP1-51908 at 1.5 ug/ml. Primary incubated for 1 hour. ATF5 antibody detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0001.jpg)
![Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221] - Western blot analysis of extracts of various cell lines, using ATF5 Rabbit mAb (NBP3-16221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/ATF5-Antibody-9Z3M0-Western-Blot-NBP3-16221-img0002.jpg "Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221] - Western blot analysis of extracts of various cell lines, using ATF5 Rabbit mAb (NBP3-16221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221] - Immunohistochemistry of paraffin-embedded human placenta using ATF5 Rabbit mAb (NBP3-16221) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-9Z3M0-Immunohistochemistry-Paraffin-NBP3-16221-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (4G5) [H00022809-M07] - Analysis of monoclonal antibody to ATF5 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ATF5-Antibody-4G5-Immunohistochemistry-Paraffin-H00022809-M07-img0002.jpg "Immunohistochemistry-Paraffin: ATF5 Antibody (4G5) [H00022809-M07] - Analysis of monoclonal antibody to ATF5 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml")
![Sandwich ELISA: ATF5 Antibody (4G5) [H00022809-M07] - Detection limit for recombinant GST tagged ATF5 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATF5-Antibody-4G5-Sandwich-ELISA-H00022809-M07-img0003.jpg)
![Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] - Image of ATF5 Antibody (9D8) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26496_rabbit-atf5-mab-9d8-2722024857338.jpg "Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] - Image of ATF5 Antibody (9D8) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: ATF5 Antibody (9D8) [NBP3-26496] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue. All lanes: ATF5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 31 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26496_rabbit-atf5-mab-9d8-28220241141367.jpg)
![Western Blot: ATF5 Antibody [NBP3-10891] - Western blot analysis of ATF5 in Human Hela. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP3-10891-img0001.jpg "Western Blot: ATF5 Antibody [NBP3-10891] - Western blot analysis of ATF5 in Human Hela. Antibody dilution at 1.0ug/ml")
![Western Blot: ATF5 Antibody [NBP3-10652] - Western blot analysis of ATF5 in Rat Stomach as a positive control. Antibody dilution at 1.0 ug/ml](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP3-10652-img0001.jpg "Western Blot: ATF5 Antibody [NBP3-10652] - Western blot analysis of ATF5 in Rat Stomach as a positive control. Antibody dilution at 1.0 ug/ml")
![ELISA: Human ATF5 ELISA Kit (Colorimetric) [NBP2-69884] - Samples were spiked with high concentrations of Human ATF5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69884_human-atf5-elisa-kit-colorimetric-13220241613046.jpg "ELISA: Human ATF5 ELISA Kit (Colorimetric) [NBP2-69884] - Samples were spiked with high concentrations of Human ATF5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ATF5 ELISA Kit (Colorimetric) [NBP2-69884] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69884_human-atf5-elisa-kit-colorimetric-43202495074.jpg)
![Western Blot: ATF5 Antibody [NBP3-10651] - Western blot analysis using NBP3-10651 on Human Liver as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP3-10651-img0001.jpg "Western Blot: ATF5 Antibody [NBP3-10651] - Western blot analysis using NBP3-10651 on Human Liver as a positive control. Antibody Titration: 0.2-1 ug/ml")