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![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Analysis in human liver and endometrium tissues. Corresponding HPD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Analysis in human liver and endometrium tissues. Corresponding HPD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human endometrium, kidney, liver and squamous epithelia using Anti-HPD antibody NBP1-89366 (A) shows similar protein distribution across tissues to independent antibody NBP2-32657 (B).](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: HPD Antibody [NBP1-89366] - Analysis using Anti-HPD antibody NBP1-89366 (A) shows similar pattern to independent antibody NBP2-32657 (B).](http://images.novusbio.com/fullsize/HPD-Antibody-Western-Blot-NBP1-89366-img0012.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human squamous epithelia shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0018.jpg)
![Western Blot: HPD Antibody [NBP1-89366] - Analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/fullsize/HPD-Antibody-Western-Blot-NBP1-89366-img0011.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0015.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0016.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0017.jpg)
![Western Blot: HPD Antibody [NBP2-32657] - Analysis using Anti-HPD antibody NBP2-32657 (A) shows similar pattern to independent antibody NBP1-89366 (B).](http://images.novusbio.com/fullsize/HPD-Antibody-Western-Blot-NBP2-32657-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HPD Antibody [NBP2-32657] - Analysis using Anti-HPD antibody NBP2-32657 (A) shows similar pattern to independent antibody NBP1-89366 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0014.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0006.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining in human liver and duodenum tissues using anti-HPD antibody. Corresponding HPD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Analysis in human liver and endometrium tissues. Corresponding HPD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human endometrium, kidney, liver and squamous epithelia using Anti-HPD antibody NBP2-32657 (A) shows similar protein distribution across tissues to independent antibody NBP1-89366 (B).](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0011.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0012.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-32657] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-32657-img0013.jpg)
![Western Blot: HPD Antibody [NBP1-31593] - Sample (30 ug of whole cell lysate)A: Hep G2 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HPD-Antibody-Western-Blot-NBP1-31593-img0002.jpg "Western Blot: HPD Antibody [NBP1-31593] - Sample (30 ug of whole cell lysate)A: Hep G2 10% SDS PAGE, antibody diluted at 1:1000.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HPD Antibody [NBP1-31593] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HPD antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31593_rabbit-polyclonal-hpd-antibody-1610202419414540.jpg)
![Immunohistochemistry: HPD Antibody [NBP3-38277] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HPD Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38277_rabbit-hpd-pab-101220241136467.jpg "Immunohistochemistry: HPD Antibody [NBP3-38277] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HPD Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: HPD Antibody [NBP3-38277] - Western blot analysis of various lysates using HPD Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38277_rabbit-hpd-pab-1012202411353071.jpg)
![Immunohistochemistry: HPD Antibody [NBP3-38277] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using HPD Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38277_rabbit-hpd-pab-1012202411404092.jpg)
![Immunocytochemistry/Immunofluorescence: HPD Antibody [NBP2-98599] - Immunofluorescence staining of HPD in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HPD polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98599-img0001.jpg "Immunocytochemistry/Immunofluorescence: HPD Antibody [NBP2-98599] - Immunofluorescence staining of HPD in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HPD polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-98599] - Immunochemical staining of human HPD in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-98599-img0003.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-98599] - Immunochemical staining of human HPD in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-98599-img0002.jpg)
![Western Blot: HPD Antibody (2F3) [H00003242-M07] - Analysis of HPD expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/HPD-Antibody-2F3-Western-Blot-H00003242-M07-img0003.jpg "Western Blot: HPD Antibody (2F3) [H00003242-M07] - Analysis of HPD expression in HeLa (Cat # L013V1).")
![ELISA: HPD Antibody (2F3) [H00003242-M07] - Detection limit for recombinant GST tagged HPD is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HPD-Antibody-2F3-ELISA-H00003242-M07-img0002.jpg)
![Western Blot: HPD Antibody [NBP1-54804] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.](http://images.novusbio.com/fullsize/HPD-Antibody-Western-Blot-NBP1-54804-img0003.jpg "Western Blot: HPD Antibody [NBP1-54804] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.")
![Western Blot: HPD Overexpression Lysate (Adult Normal) [NBL1-11691] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HPD.](http://images.novusbio.com/fullsize/HPD-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11691-img0002.jpg "Western Blot: HPD Overexpression Lysate (Adult Normal) [NBL1-11691] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HPD.")
![SDS-Page: Recombinant Human HPD His Protein [NBP1-49443] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human HPD His Protein-SDS-Page-NBP1-49443-img0004.jpg "SDS-Page: Recombinant Human HPD His Protein [NBP1-49443] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Immunohistochemistry-Paraffin: HPD Antibody [NBP2-99592] - Immunochemical staining of human HPD in human kidney with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HPD-Antibody-Immunohistochemistry-Paraffin-NBP2-99592-img0001.jpg "Immunohistochemistry-Paraffin: HPD Antibody [NBP2-99592] - Immunochemical staining of human HPD in human kidney with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.")
![ELISA: Human HPD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31020] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31020_human-hpd-ready-to-use-elisa-kit-colorimetric-206202415294011.png "ELISA: Human HPD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31020] - Standard Curve Reference")
![ELISA: Human HPD ELISA Kit (Colorimetric) [NBP3-31019] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31019_human-hpd-elisa-kit-colorimetric-206202415361337.png "ELISA: Human HPD ELISA Kit (Colorimetric) [NBP3-31019] - Standard Curve Reference")