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![Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleolus by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13283-img0004.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP3-13283-img0002.jpg)
![Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRM antibody (NBP3-13283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13283-img0003.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP3-13283-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-Immunocytochemistry-Immunofluorescence-NBP3-13725-img0004.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - Mouse brain. BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-Immunohistochemistry-Paraffin-NBP3-13725-img0002.jpg)
![BRM Antibody (HL1115) [NBP3-13725] - Rat brain. SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-NBP3-13725-img0003.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - BRM antibody [HL1115] detects BRM protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13725_rabbit-brm-mab-hl1115-91020242011423.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - BRM antibody [HL1115] detects BRM protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13725_rabbit-brm-mab-hl1115-9102024204125.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: BRM Antibody (HL1115) [NBP3-13725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA2 antibody [HL1115] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13725_rabbit-brm-mab-hl1115-1610202419405785.jpg)
![Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90015-img0007.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0011.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0008.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0009.jpg)
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human pancreas shows weak to moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-90015-img0010.jpg)
![Western Blot: BRM Antibody (PSH01-15) [NBP3-32957] - Western blot analysis of BRM on different lysates with Rabbit anti-BRM antibody (NBP3-32957) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: MCF7 cell lysate Lane 5: COS-1 cell lysate Lane 6: NCCIT cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 181 kDa Observed band size: 200 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32957) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32957_rabbit-brm-mab-psh01-15-288202412461625.jpg "Western Blot: BRM Antibody (PSH01-15) [NBP3-32957] - Western blot analysis of BRM on different lysates with Rabbit anti-BRM antibody (NBP3-32957) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: MCF7 cell lysate Lane 5: COS-1 cell lysate Lane 6: NCCIT cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 181 kDa Observed band size: 200 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32957) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BRM Antibody (PSH01-15) [NBP3-32957] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-BRM antibody (NBP3-32957) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32957) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32957_rabbit-brm-mab-psh01-15-29820241492642.jpg)
![Flow Cytometry: BRM Antibody (PSH01-15) [NBP3-32957] - Flow cytometric analysis of MCF7 cells labeling BRM. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32957, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32957_rabbit-brm-mab-psh01-15-29202416453232.jpg)
![Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/BRM-Antibody-NB100-55309-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. For blotting immunoprecipitated SMARCA2/BRM, NB100-55309 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunoprecipitation-NB100-55309-img0004.jpg)
![QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRM-Antibody-QcWestern-NB100-55309-img0005.jpg)
![Western Blot: BRM Antibody [NBP1-80042] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP1-80042-img0003.jpg "Western Blot: BRM Antibody [NBP1-80042] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: BRM Antibody [NBP1-80042] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP1-80042-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BRM Antibody (2D12) [H00006595-M06] - Analysis of monoclonal antibody to SMARCA2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/BRM-Antibody-2D12-Immunocytochemistry-Immunofluorescence-H00006595-M06-img0002.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody (2D12) [H00006595-M06] - Analysis of monoclonal antibody to SMARCA2 on HeLa cell. Antibody concentration 10 ug/ml")
![ELISA: BRM Antibody (2D12) [H00006595-M06] - Detection limit for recombinant GST tagged SMARCA2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-2D12-ELISA-H00006595-M06-img0001.jpg)
![Western Blot: BRM Antibody [NB100-55308] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55308 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55309, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/BRM-Antibody-NB100-55308-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BRM Antibody [NB100-55308] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55308 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55309, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![SDS-Page: Recombinant Human BRM Protein [H00006595-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BRM-Protein-SDS-Page-H00006595-Q01-img0002.jpg "SDS-Page: Recombinant Human BRM Protein [H00006595-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: BRM Antibody [NB100-55307] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate. SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NB100-55307-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BRM Antibody [NB100-55307] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate. SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309.")