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![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Analysis in human testis and endometrium tissues. Corresponding HspA1L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-92012-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Analysis in human testis and endometrium tissues. Corresponding HspA1L RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HspA1L Antibody [NBP1-92012] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-92012-img0008.jpg)
![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-92012-img0016.jpg)
![Western Blot: HspA1L Antibody [NBP1-92012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-92012-img0006.jpg)
![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-92012-img0013.jpg)
![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-92012-img0014.jpg)
![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-92012-img0015.jpg)
![Western Blot: HspA1L Antibody [NBP1-33022] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-33022-img0011.jpg "Western Blot: HspA1L Antibody [NBP1-33022] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HspA1L Antibody [NBP1-33022] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] diluted at 1:400. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33022-img0007.jpg)
![Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-33022] - Paraffin-embedded H661 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunohistochemistry-Paraffin-NBP1-33022-img0003.jpg)
![Western Blot: HspA1L Antibody [NBP1-33022] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-33022-img0004.jpg)
![Western Blot: HspA1L Antibody [NBP1-33022] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-33022-img0006.jpg)
![Western Blot: HspA1L Antibody [NBP1-33022] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-33022-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HspA1L Antibody [NBP1-33022] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33022-img0002.jpg)
![Western Blot: HspA1L Antibody (7H6) [H00003305-M02] - HSPA1L monoclonal antibody (M02), clone 7H6. Analysis of HSPA1L expression in PC-12.](http://images.novusbio.com/fullsize/HspA1L-Antibody-7H6-Western-Blot-H00003305-M02-img0010.jpg "Western Blot: HspA1L Antibody (7H6) [H00003305-M02] - HSPA1L monoclonal antibody (M02), clone 7H6. Analysis of HSPA1L expression in PC-12.")
![Immunohistochemistry-Paraffin: HspA1L Antibody (7H6) [H00003305-M02] - Analysis of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HspA1L-Antibody-7H6-Immunohistochemistry-Paraffin-H00003305-M02-img0007.jpg)
![Western Blot: HspA1L Antibody (7H6) [H00003305-M02] - HSPA1L monoclonal antibody (M02), clone 7H6 Analysis of HSPA1L expression in HeLa.](http://images.novusbio.com/fullsize/HspA1L-Antibody-7H6-Western-Blot-H00003305-M02-img0009.jpg)
![Proximity Ligation Assay: HspA1L Antibody (7H6) [H00003305-M02] - Analysis of protein-protein interactions between MAP3K7 and HSPA1L. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-HSPA1L mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HspA1L-Antibody-7H6-Proximity-Ligation-Assay-H00003305-M02-img0005.jpg)
![Sandwich ELISA: HspA1L Antibody (7H6) [H00003305-M02] - Detection limit for recombinant GST tagged HSPA1L is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HspA1L-Antibody-7H6-Sandwich-ELISA-H00003305-M02-img0008.jpg)
![Western Blot: HspA1L Antibody [NBP1-32587] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA1L antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-32587-img0006.jpg "Western Blot: HspA1L Antibody [NBP1-32587] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA1L antibody diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: HspA1L Antibody [NBP1-32587] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32587-img0003.jpg)
![Western Blot: HspA1L Antibody [NBP1-32587] - (30 ug whole cell lysate) A: H1299 B: HeLa S3 C: Hep G2 D: MOLT4 E: Raji 7.5% SDS PAGE; antibody diluted at 1:1000](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-32587-img0001.jpg)
![Western Blot: HspA1L Antibody [NBP1-32587] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA1L antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-32587-img0004.jpg)
![Western Blot: HspA1L Antibody [NBP1-32587] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HspA1L antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-32587-img0005.jpg)
![Western Blot: HspA1L Antibody (7D3-R) [NBP3-32447] - Western blot analysis of HspA1L on different lysates with Mouse anti-HspA1L antibody (NBP3-32447) at 1/2,000 dilution. Lane 1: NCI-H1299 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Mouse testis tissue lysate (40 ug/Lane) Lane 4: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: Lane 1-2: 5 minutes; Lane 3-4: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32447) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32447_mouse-hspa1l-mab-7d3-r-278202415212486.jpg "Western Blot: HspA1L Antibody (7D3-R) [NBP3-32447] - Western blot analysis of HspA1L on different lysates with Mouse anti-HspA1L antibody (NBP3-32447) at 1/2,000 dilution. Lane 1: NCI-H1299 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Mouse testis tissue lysate (40 ug/Lane) Lane 4: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: Lane 1-2: 5 minutes; Lane 3-4: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32447) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HspA1L Antibody (7D3-R) [NBP3-32447] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-HspA1L antibody (NBP3-32447) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32447) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32447_mouse-hspa1l-mab-7d3-r-298202413585525.jpg)
![Immunocytochemistry/ Immunofluorescence: HspA1L Antibody (7D3-R) [NBP3-32447] - Immunocytochemistry analysis of HeLa cells labeling HspA1L with Mouse anti-HspA1L antibody (NBP3-32447) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HspA1L antibody (NBP3-32447) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32447_mouse-hspa1l-mab-7d3-r-29202410234231.jpg)
![Western Blot: HspA1L Antibody (2-1#) [NBP3-41479] - Sample: Human HepG2 cell lysate; Primary Ab: 3ug/ml NBP3-41479; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41479_mouse-hspa1l-mab-2-1-612202421331692.jpg "Western Blot: HspA1L Antibody (2-1#) [NBP3-41479] - Sample: Human HepG2 cell lysate; Primary Ab: 3ug/ml NBP3-41479; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: HspA1L Antibody (2-1#) [NBP3-41479] - Sample: Human Raji cell lysate; Primary Ab: 3ug/ml NBP3-41479; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41479_mouse-hspa1l-mab-2-1-612202421175924.jpg)
![Western Blot: HspA1L Antibody (2-1#) [NBP3-41479] - Sample: Human Hela cell lysate; Primary Ab: 3ug/ml NBP3-41479; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41479_mouse-hspa1l-mab-2-1-61220242195745.jpg)
![Western Blot: HspA1L Antibody (C3) [NBP3-41562] - Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Human Saliva; Lane4: Porcine Cerebrum lysate; Lane5: Rat Testis lysate Primary Ab: 0.02ug/ml NBP3-41562; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41562_mouse-hspa1l-mab-c3-61220242124324.jpg "Western Blot: HspA1L Antibody (C3) [NBP3-41562] - Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Human Saliva; Lane4: Porcine Cerebrum lysate; Lane5: Rat Testis lysate Primary Ab: 0.02ug/ml NBP3-41562; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: HspA1L Antibody (6#) [NBP3-41954] - Sample: Recombinant HSPA1L, Rat.](http://images.novusbio.com/fullsize/nbp3-41954_mouse-hspa1l-mab-6-612202421373082.jpg "Western Blot: HspA1L Antibody (6#) [NBP3-41954] - Sample: Recombinant HSPA1L, Rat.")
![Western Blot: HspA1L Antibody [NBP1-56938] - Recommended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-56938-img0004.jpg "Western Blot: HspA1L Antibody [NBP1-56938] - Recommended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate")
![Western Blot: HspA1L Antibody [NBP1-56938] - Sample Type: 1. Lamprey CNS (20ug) 2. Rat Brain Lysate (20ug) Primary Dilution: 1:1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary Dilution: 1:4000](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-56938-img0002.jpg)
![Western Blot: HspA1L Antibody [NBP1-56938] - Sample Tissue: Human MDA-MB-435s Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/HspA1L-Antibody-Western-Blot-NBP1-56938-img0003.jpg)
![Western Blot: HspA1L Antibody (C1) [NBP3-41625] - Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Rat Testis lysate Primary Ab: 0.5ug/ml NBP3-41625; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41625_mouse-hspa1l-mab-c1-612202421395344.jpg "Western Blot: HspA1L Antibody (C1) [NBP3-41625] - Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Rat Testis lysate Primary Ab: 0.5ug/ml NBP3-41625; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: HspA1L Antibody (1B5) [H00003305-M01] - HSPA1L monoclonal antibody (M01), clone 1B5. Analysis of HSPA1L expression in PC-12.](http://images.novusbio.com/fullsize/HspA1L-Antibody-1B5-Western-Blot-H00003305-M01-img0004.jpg "Western Blot: HspA1L Antibody (1B5) [H00003305-M01] - HSPA1L monoclonal antibody (M01), clone 1B5. Analysis of HSPA1L expression in PC-12.")
![Western Blot: HspA1L Antibody (1B5) [H00003305-M01] - HSPA1L monoclonal antibody (M01), clone 1B5. Analysis of HSPA1L expression in HeLa.](http://images.novusbio.com/fullsize/HspA1L-Antibody-1B5-Western-Blot-H00003305-M01-img0003.jpg)
![SDS-Page: Recombinant Human HspA1L Protein [H00003305-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003305-P01-1.jpg "SDS-Page: Recombinant Human HspA1L Protein [H00003305-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")