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SREBP1 Antibody (2A4) - BSA Free

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Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear ...read more
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Genetic Strategies: Knockout Validated: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the expression of Nrf2‐related signalling proteins in OA (660 μmol/L) & PA (330 μmol/L)‐induced HepG2 cells. GEN was added into HepG2 ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - KO & OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. (A) Total cellular TG content of SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (B) HPLC-MS ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Construction of knockout (KO) & overexpression (OE) cell lines. (A) The sequencing results of KO positive clone cells; (B) The messenger RNA (mRNA) level of ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h ...read more
Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the protein expression of Nrf2, ACC & mTORC signalling pathways in tyloxapol‐induced mice. GEN (50, 75, 100 mg/kg) was administered to WT ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHaSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clone
2A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

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SREBP1 Antibody (2A4) - BSA Free Summary

Immunogen
6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Localization
Cytoplasmic and nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SREBF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Frozen
  • Knockout Validated
  • Simple Western 1:12.5
  • Western Blot 1-2ug/ml
Application Notes
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The use of this antibody in IHC paraffin embedded tissue has been questionable.
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB600-582 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SREBP1 Antibody (2A4) - BSA Free

  • bHLHd1
  • Class D basic helix-loop-helix protein 1
  • SREBF1
  • SREBP 1c
  • SREBP1
  • SREBP-1
  • SREBP1bHLHd1SREBP-1c
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-582
Species: Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa
Applications: WB, Simple Western, IHC, KO

Publications for SREBP1 Antibody (NB600-582)(65)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster.

We have publications tested in 3 applications: IHC-F, Simple Western, WB.


Filter By Application
IHC-F
(1)
Simple Western
(1)
WB
(48)
All Applications
Filter By Species
Human
(13)
Mouse
(25)
Rat
(6)
Bovine
(1)
Canine
(1)
Chicken
(2)
Hamster
(2)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using NB600-582 Applications Species
Saito K, Sekiya M, Kainoh K et al. Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activity The Journal of biological chemistry 2023-06-05 [PMID: 37286039] (WB, Human) WB Human
Srinivasan MP, Bhopale KK, Caracheo AA et al. Dysregulated pancreatic lipid phenotype, inflammation and cellular injury in a chronic ethanol feeding model of hepatic alcohol dehydrogenase-deficient deer mice Life sciences 2023-06-01 [PMID: 37030615] (WB, Mouse) WB Mouse
Tajima O, Fujita Y, Ohmi Y et al. Ganglioside GM3 prevents high fat diet-induced hepatosteatosis via attenuated insulin signaling pathway PloS one 2023-02-24 [PMID: 36827398] (WB, Mouse) WB Mouse
Huang CY, Chen HW, Lo CW et al. Luteolin ameliorates palmitate-induced lipotoxicity in hepatocytes by mediating endoplasmic reticulum stress and autophagy Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023-01-01 [PMID: 36509263]
Chen YJ, Lo YH, Chen YT et al. Magnesium lithospermate B improves metabolic changes in high-fat diet-fed rats with metabolic syndrome Journal of Functional Foods. 2015-04-01 (WB, Rat)

Details:
SREBP1 antibody was used for WB application on lysates of liver tissue from rats that were fed different diet regimns as - normal diet control/NC, high-fat diet control/HC or HC+ MLB/Magnesium lithospermate B (MLB5-20/HC+5-20?mg/kg/day of MLB). Primary antibody was used at 1:1000 dilution in 5% skim milk and detected with HRP-conjugated secondary antibody - chemiluminescent HRP substrate (data shown in Fig. 4A).
WB Rat
Cook J. Toward a Mechanistic Understanding of Hepatic Insulin Action and Resistance. Thesis. 2014-01-01 (WB, Mouse) WB Mouse
Matthew Grove, Hyukmin Kim, Shuhuan Pang, Jose Paz Amaya, Guoqing Hu, Jiliang Zhou, Michel Lemay, Young-Jin Son, Klaus-Armin Nave, Timothy E Behrens TEAD1 is crucial for developmental myelination, Remak bundles, and functional regeneration of peripheral nerves eLife 2024-03-08 [PMID: 38456457]
Liu M, Liu L, Guo H et al. Dominant-negative HNF1? mutant promotes liver steatosis and inflammation by regulating hepatic complement factor D iScience 2023-09-01 [PMID: 37841581] (WB, Mouse) WB Mouse
Yang M, Mariano J, Su R et al. SARS-CoV-2 papain-like protease (PLpro) plays multiple roles in regulating cellular proteins in the endoplasmic reticulum The Journal of biological chemistry 2023-10-12 [PMID: 37838170] (WB, Human) WB Human
Zhu Y, Lei L, Wang X et al. Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver Journal of dairy science 2023-06-06 [PMID: 37291038] (WB, Bovine) WB Bovine
Show All 65 Publications.

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Product General Protocols

View specific protocols for SREBP1 Antibody (NB600-582): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
    • We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
  2. I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
    • Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SREBP1 Products

Research Areas for SREBP1 Antibody (NB600-582)

Find related products by research area.

Blogs on SREBP1.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ...  Read full blog post.

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Bioinformatics

Gene Symbol SREBF1
Entrez
Uniprot