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Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

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Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 4‑20 pg/mL.
Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ...read more
1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
211-TB-010
Carrier Free
211-TBB-010/CF
Carrier Free
211-TBB-050/CF
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Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
Source
E. coli-derived human Lymphotoxin-alpha/TNF-beta protein
Leu35-Leu205, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
LTA
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-19 kDa, reducing conditions
Publications
Read Publications using
211-TBB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).

  1. Nedwin, G.E. et al. (1985) J. Cell. Biochem. 29:171.
  2. Gray, P.W. et al. (1984) Nature 312:721.
  3. Kobayashi, Y. et al. (1986) J. Biochem. 100:727.
  4. Warzocha, K. et al. (1995) Eur. Cytokine Netw. 6:83.
  5. McCarthy, D.D. et al.(2006) Immunol. Res. 35:41.
  6. Drutskaya, M.S. et al. (2010) IUBMB Life 62:283.
  7. De Togni, P. et al. (1994) Science 264:703.
  8. Seleznik, G.M. et al. (2014) Cytokine Growth Factor Rev. 25:125.
  9. Mapara, M.Y. et al. (1994) Int. J. Cancer 58:248.
  10. Chiang, E.Y. et al. (2009) Nat. Med. 15:766.

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Recombinant Human
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211-TBB
Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
Species: Hu
Applications: Bioactivity

Publications for Lymphotoxin-alpha/TNF-beta (211-TBB)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(8)
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Human
(6)
Mouse
(2)
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Showing Publications 1 - 8 of 8.
Publications using 211-TBB Applications Species
M Fellermann, C Huchler, L Fechter, T Kolb, F Wondany, D Mayer, J Michaelis, S Stenger, K Mellert, P Möller, TFE Barth, S Fischer, H Barth Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells Toxins (Basel), 2020-09-01;12(9):. 2020-09-01 [PMID: 32883045] (Bioassay, Human) Bioassay Human
TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020-01-30;16(1):e1008261. 2020-01-30 [PMID: 31999807] (Bioassay, Human) Bioassay Human
JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018-03-01;0(0):. 2018-03-01 [PMID: 29496671] (Bioassay, Human) Bioassay Human
Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta. Eur J Immunol, 2015-04-17;45(6):1783-93. 2015-04-17 [PMID: 25773885] (Bioassay, Human) Bioassay Human
Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2014-12-23;290(7):4022-37. 2014-12-23 [PMID: 25538244] (Bioassay, Human) Bioassay Human
Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice. Am J Pathol, 2014-04-01;184(4):1152-66. 2014-04-01 [PMID: 24655377] (Bioassay, Mouse) Bioassay Mouse
Vang , Amanda G, Housley , William, Dong , Hongli, Basole , Chaitali, Ben-Sasson , Shlomo Z, Kream , Barbara, Epstein , Paul M, Clark , Robert B, Brocke , Stefan Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac. Biochem J, 2013-12-15;456(3):463-73. 2013-12-15 [PMID: 24007532] (Bioassay, Mouse) Bioassay Mouse
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol LTA
Uniprot