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RALBP1 Antibody (2A1)

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Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1 Analysis of RALBP1 expression in HeLa.
Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1 Analysis of RALBP1 expression in HeLa.
Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - Analysis of RALBP1 transfected lysate using anti-RALBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RALBP1 MaxPab rabbit polyclonal ...read more
Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1. Analysis of RALBP1 expression in C32.
ELISA: RALBP1 Antibody (2A1) [H00010928-M02] - Detection limit for recombinant GST tagged RALBP1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
2A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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RALBP1 Antibody (2A1) Summary

Immunogen
RALBP1 (AAH13126.1, 1 a.a. ~ 655 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQISNRVLYVFFTHVQELFGNVVLKQVMKPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAREQPKAGKEPAKPSPSRDRKETSI
Localization
Cytoplasmic
Specificity
RALBP1 - ralA binding protein 1
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RALBP1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1:10-1:1000
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Publications
Read Publications using H00010928-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RALBP1 Antibody (2A1)

  • Dinitrophenyl S-glutathione ATPase
  • ralA binding protein 1
  • ralA-binding protein 1,76 kDa Ral-interacting protein
  • RalBP1
  • Ral-interacting protein 1
  • RIP
  • RIP1
  • RLIP1
  • RLIP76DNP-SG ATPase

Background

The receptor interacting protein kinase 1 (RIP1) is essential for the activation of nuclear factor kappaB (NF-kappaB) by tumor necrosis factor alpha (TNFalpha). RIP1 can be polyubiquitinated at Lys 377, which is required for the activation of IkappaB kinase (IKK) and NF-kappaB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RALBP1 Antibody (H00010928-M02)(8)


Showing Publications 1 - 8 of 8.
Publications using H00010928-M02 Applications Species
M Kobayashi, H Katayama, E Irajizad, JV Vykoukal, JF Fahrmann, DL Kundnani, CY Yu, Y Cai, FC Hsiao, WL Yang, Z Lu, J Celestino, JP Long, KA Do, KH Lu, JJ Ladd, N Urban, RC Bast, SM Hanash Proteome Profiling Uncovers an Autoimmune Response Signature That Reflects Ovarian Cancer Pathogenesis Cancers (Basel), 2020-02-19;12(2):. 2020-02-19 [PMID: 32092936]
Tazat K, Harsat M, Goldshmid-Shagal A et al. Dual effects of Ral-activated pathways on p27 localization and TGF-b signaling. Mol Biol Cell. 2013-04-10 [PMID: 23576547]
Neel NF, Rossman KL, Martin TD et al. The RalB Small GTPase Mediates Invadopodia Formation Through a GTPase Activating Protein-independent Function of the RalBP1/RLIP76 Effector. Mol Cell Biol. 2012-02-13 [PMID: 22331470]
Fillatre J, Delacour D, Van Hove L et al. Dynamics of the subcellular localization of RalBP1/RLIP through the cell cycle: the role of targeting signals and of protein-protein interactions. FASEB J. 2012-02-08 [PMID: 22319010]
Kashatus DF, Lim K-H, Brady DC et al. RALA and RALBP1 regulate mitochondrial fission at mitosis. Nat Cell Biol. 2011-08-07 [PMID: 21822277]
Kidd AR 3rd, Snider JL, Martin TD et al. Ras-Related Small GTPases RalA and RalB Regulate Cellular Survival After Ionizing Radiation. Int J Radiat Oncol Biol Phys. 2010-07-07 [PMID: 20619549]
Herlevsen MC, Theodorescu D. Mass spectroscopic phosphoprotein mapping of Ral binding protein 1 (RalBP1/Rip1/RLIP76). Biochem Biophys Res Commun;362(1):56-62. 2007-10-12 [PMID: 17706599]
Smith SC, Oxford G, Baras AS et al. Expression of Ral GTPases, Their Effectors, Activators in Human Bladder Cancer. Clin Cancer Res;13(13):3803-13. 2007-07-01 [PMID: 17606711]

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Bioinformatics

Gene Symbol RALBP1
Entrez
OMIM
Uniprot