VPS34 Antibody

VPS34 (vacuolar protein sorting 34) is a member of PI3K family of lipid kinases, all of which phosphorylate the 3' hydroxy position of phosphatidylinositol ring. VPS34 is ubiquitously expressed with highest expression in skeletal muscles and it localizes to midbody/late endosomes in cells. VPS34 forms a complexes with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14, and the presence of UVRAG/ATG14 are mutually exclusive in this complex. It forms complex with PIK3R4/PIK3CB also, and interacts with RAB7A in the presence of PIK3R4. VPS34 regulates vesicular trafficking in the endosomal/lysosomal system, where it is involved in the recruitment of proteins containing phosphatidylinositol 3-phosphate (PtdIns3P) binding domains to intracellular membranes. As PI3K complex's catalytic subunit, VPS34 mediates formation of PtdIns3P which is involved in initiation and maturation of autophagosomes. Moreover, VPS34 is implicated in transporting lysosomal enzyme precursors to lysosomes, required for abcission step in cytokinesis and transport from early to late endosomes. Vps34 has also been involved in other signalling processes such as nutrient sensing in mTOR pathway in mammalian cells and trimeric G-protein signalling to MAPK in yeast.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, WB.

Showing Publications 1 - 8 of 8.

Publications using NB110-87320	Applications	Species
Yang D, Wu X, Wang W et al. Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy Advanced Science 2022-01-01 [PMID: 34783461] (WB)	WB
Rijal R, Cadena LA, Smith MR et al. Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells Proceedings of the National Academy of Sciences of the United States of America 2020-12-15 [PMID: 33268492]
Meena N, Ralston E, Raben N, Puertollano R Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease Mol Ther Methods Clin Dev 2020-07-17 [PMID: 32671132] (FLOW, Human)	FLOW	Human
Lin M W, Lin C C et al. Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy. Antioxidants (Basel) 2019-12-31 [PMID: 31906147] (WB, Mouse)	WB	Mouse
Campa CC, Margaria JP, Derle A et al. Rab11 activity and PtdIns(3)P turnover removes recycling cargo from endosomes Nat. Chem. Biol. 2018-06-18 [PMID: 29915378] (WB)	WB
Brigger D, Schlafli AM, Garattini E, Tschan MP. Activation of RARa induces autophagy in SKBR3 breast cancer cells and depletion of key autophagy genes enhances ATRA toxicity. Cell Death Dis 2015-08-28 [PMID: 26313912] (WB, Human)	WB	Human
Shi JM, Bai LL, Zhang DM et al. Saxifragifolin D induces the interplay between apoptosis and autophagy in breast cancer cells through ROS-dependent endoplasmic reticulum stress. Biochem Pharmacol 2013-01-21 [PMID: 23348250] (WB, Human)	WB	Human
Huang Y, Hou JK, Chen TT et al. PML-RAR alpha enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway. Autophagy. 2011-10-01 [PMID: 21673516] (WB, Human)	WB	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Naoya Nakai	WB	Mouse	07/27/2017	View Summary Application Western Blot Sample Tested Skeletal muscle satellite cells (cultured in vitro) Species Mouse

Array NB110-87320

• VPS34 Antibodies
• VPS34 Lysate
• VPS34 Proteins