![Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Western-Blot-NBP2-22410-img0002.jpg "Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S187-7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN |
| Localization | Membrane |
| Specificity | Detects approx 200kDa. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NALCN |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of NALCN Antibody was sufficient for detection of NALCN in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP asthe secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0003.jpg "Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0001.jpg "Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.")
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGCNL1Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0004.jpg "Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGCNL1Antibody. (D) Composite.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")