Home » VCAM-1/CD106 » VCAM-1/CD106 Antibodies » VCAM-1/CD106 Antibody (112734) [Alexa Fluor® 750]
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VCAM-1/CD106 Antibody (112734) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
112734
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6432U-100UG
Alexa Fluor 405
FAB6432V-100UG
Alexa Fluor 488
FAB6432G-100UG
Alexa Fluor 532
FAB6432X
Alexa Fluor 594
FAB6432T-100UG
Alexa Fluor 647
FAB6432R-100UG
Alexa Fluor 700
FAB6432N
Allophycocyanin/Cy7
FAB6432APCCY7
Biotin
FAB6432B
DyLight 350
FAB6432D
DyLight 405
FAB6432E
DyLight 488
FAB6432K
DyLight 550
FAB6432L
DyLight 594
FAB6432M
DyLight 650
FAB6432W
DyLight 680
FAB6432Y
DyLight 755
FAB6432Z
FITC
FAB6432F
HRP
FAB6432H
Janelia Fluor 549
FAB6432I
Janelia Fluor 646
FAB6432J
PE/Atto594
FAB6432PEATT594
PE/Cy5.5
FAB6432PECY55
PE/Cy7
FAB6432PECY7
Phycoerythrin
FAB6432P
Unconjugated
MAB6432-100
mFluor Violet 450 SE
FAB6432MFV450
mFluor Violet 500 SE
FAB6432MFV500
mFluor Violet 610 SE
FAB6432MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6432-100
Carrier Free
FAB6432N
Carrier Free
FAB6432P
Carrier Free
MAB6432-SP
Carrier Free
MAB6432-500
Carrier Free
MAB6432-100
Carrier Free
FAB6432V-100UG
Carrier Free
FAB6432U-100UG
Carrier Free
FAB6432T-100UG
Carrier Free
FAB6432R-100UG
Carrier Free
FAB6432G-100UG
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VCAM-1/CD106 Antibody (112734) [Alexa Fluor® 750] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1
Phe25-Glu698 (predicted)
Accession # P29533
Specificity
Detects recombinant mouse (rm) VCAM-1 in Western blots and ELISAs. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human VCAM-1, rmICAM-1, rmE-Selectin, rmL-Selectin or rmP-Selectin.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
VCAM1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody (112734) [Alexa Fluor® 750]

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta , IL-4, TNF-alpha and IFN-gamma . VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VCAM1
Uniprot