Human UCP3 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human UCP3 in samples. No Significant cross-reactivity or interference between Human UCP3 and analogues was observed. |
Standard Curve Range |
78.13 - 5000 pg/mL |
Sensitivity |
46.88 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.44 |
Intra-Assay |
CV% < 4.58 |
Spike Recovery |
87-108% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
UCP3 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human UCP3 ELISA Kit (Colorimetric)
Background
The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissue of mammals. UCP 1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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