![Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.Lane 1: UBR2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UBR2-Antibody-4G4-Western-Blot-H00023304-M01-img0008.jpg "Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.Lane 1: UBR2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, KD |
| Clone | 4G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | UBR2 (NP_056070, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCG |
| Specificity | UBR2 - ubiquitin protein ligase E3 component n-recognin 2 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UBR2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of monoclonal antibody to UBR2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/UBR2-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00023304-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of monoclonal antibody to UBR2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( Cat # H00023304-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UBR2 monoclonal antibody (M01), clone 4G4 (Cat # H00023304-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/UBR2-Antibody-4G4-Western-Blot-H00023304-M01-img0006.jpg "Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( Cat # H00023304-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UBR2 monoclonal antibody (M01), clone 4G4 (Cat # H00023304-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: UBR2 Antibody (4G4) [H00023304-M01] - Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/UBR2-Antibody-4G4-Sandwich-ELISA-H00023304-M01-img0007.jpg "Sandwich ELISA: UBR2 Antibody (4G4) [H00023304-M01] - Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.")
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