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UbcH2/UBE2H Antibody (3C4-1A2)

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Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - UBE2H monoclonal antibody (M01), clone 3C4-1A2 Analysis of UBE2H expression in HeLa.
Immunocytochemistry/ Immunofluorescence: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - Analysis of monoclonal antibody to UBE2H on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - Analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane ...read more
Sandwich ELISA: UbcH2/UBE2H Antibody (3C4-1A2) [H00007328-M01] - Detection limit for recombinant GST tagged UBE2H is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3C4-1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

UbcH2/UBE2H Antibody (3C4-1A2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
UBE2H (AAH06277, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL
Specificity
UBE2H - ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBE2H
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UbcH2/UBE2H Antibody (3C4-1A2)

  • E2-14K
  • E2-20K
  • EC 6.3.2.19
  • UBC8
  • UBC8E2-20K
  • UBCH
  • UbcH2
  • UBE2H
  • Ubiquitin carrier protein H
  • ubiquitin-conjugating enzyme E2 H
  • Ubiquitin-conjugating enzyme E2-20K
  • ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
  • ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
  • Ubiquitin-protein ligase H

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2H
Entrez
OMIM
Uniprot