![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Western blot analysis of Transgelin/TAGLN/SM22 alpha on Caco-2 cell lysates with Rabbit anti-Transgelin/TAGLN/SM22 alpha antibody (NBP3-32992) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32992_rabbit-transgelin-tagln-sm22-alpha-mab-je60-72-288202412493464.jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Western blot analysis of Transgelin/TAGLN/SM22 alpha on Caco-2 cell lysates with Rabbit anti-Transgelin/TAGLN/SM22 alpha antibody (NBP3-32992) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | JE60-72 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human Transgelin/TAGLN/SM22 alpha aa 126-175 / 201. (Uniprot: Q01995) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TAGLN |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TAGLN |
![Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Transgelin/TAGLN/SM22 alpha antibody (NBP3-32992) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32992) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32992_rabbit-transgelin-tagln-sm22-alpha-mab-je60-72-298202414115439.jpg "Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Transgelin/TAGLN/SM22 alpha antibody (NBP3-32992) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32992) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Flow cytometric analysis of Caco-2 cells labeling Transgelin/TAGLN/SM22 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32992, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32992_rabbit-transgelin-tagln-sm22-alpha-mab-je60-72-29202416372148.jpg "Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (JE60-72) [NBP3-32992] - Flow cytometric analysis of Caco-2 cells labeling Transgelin/TAGLN/SM22 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32992, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images2/Myocardin_MAB4028_Western_Blot_6147.jpg)
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images/mab4028_human-mouse-myocardin-mab-clone-355521-412024125217.jpg)
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images/mab4028_human-mouse-myocardin-mab-clone-355521-41202412505317.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")