![Western Blot: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Western-Blot-NBP2-34602-img0007.jpg "Western Blot: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, CyTOF-ready |
| Clone | DF1513 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32945). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Additional Information | Clone DF1513 was used by HLDA to establish CD designation. |
| Immunogen | KG1 acute myeloid leukemia cell line |
| Localization | Cell surface |
| Specificity | It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFRC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TfR (Transferrin R) Antibody (NBP2-34602)Find related products by research area.
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Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TFRC |
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34602-img0006.jpg "Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34602-img0008.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).")
![Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Simple-Western-NBP2-34602-img0004.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Simple-Western-NBP2-34602-img0005.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.")
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