Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Sodium Potassium ATPase Beta 1 (NKA-beta1), member of X(+)/potassium ATPases subunit beta family, is a single-pass type II membrane protein (transmembrane enzyme) responsible for active reciprocal transport of Na+/K+ ions across plasma membranes, and it contains a larger alpha subunit which catalyze ATP hydrolysis and a smaller beta subunit which is the non-catalytic component of the active enzyme. The alpha subunit (113 kD) contains the binding sites for ATP, Na+ ions, K+ ions, and allosteric sites for inhibitors and activators, and is capable of catalyzing ATP hydrolysis as an essential energy source to transport 3 Na+ ions out of the cell and 2 K+ ions into the cell against membrane ion gradients. The beta subunit (35 kD) is a glycoprotein with isoforms beta1, beta2 and beta3 which are non-catalytic in nature because they lack binding sites for ATP or Na+/K+ ions. In fact, beta subunit is a specific chaperone that assists the biogenesis and corrects membrane insertion of newly synthesized NKA. Moreover, it exerts allosteric activities and by altering the apparent affinity of Na+ /K+ ions as well as by enhancing enzyme turnover rate, NKA-beta1 remotely controls the catalytic function of the enzyme through JY2948-beta1-alpha1 interaction complex. Furthermore, NKA-beta1 may dictate functional NKA concentrations in caveolae, can promote translation efficiency of the alpha subunit and mediate alpha/beta assembly as well as beta-beta homo-oligomerization, and is essential to epithelial polarization, suppression of invasion, and cell motility.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Rabbit, Xenopus.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NB300-147	Applications	Species
Harich, OO;Gavriliuc, OI;Ordodi, VL;Tirziu, A;Paunescu, V;Panaitescu, C;Bojin, MF; In Vitro Study of the Multimodal Effect of Na+/K+ ATPase Blocker Ouabain on the Tumor Microenvironment and Malignant Cells Biomedicines 2023-08-05 [PMID: 37626702] (ICC/IF)	ICC/IF
Sampaio L, Shiju T, Hilgert G Et al. Descemet's membrane injury and regeneration, and posterior corneal fibrosis in rabbits Experimental Eye Research 2021-11-01 [PMID: 34736886] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Soltysik K, Ohsaki Y, Tatematsu T et al. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner The Journal of cell biology 2021-01-04 [PMID: 33315072]
Shimamoto S, Nakashima K, Kamimura R et al. Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes Gen. Comp. Endocrinol. 2019-07-26 [PMID: 31356813] (WB, Chicken)	WB	Chicken
Tokhtaeva E, Sun H, Deiss-Yehiely N et al. FXYD5 O-glycosylated ectodomain impairs adhesion by disrupting cell-cell trans-dimerization of Na,k-ATPase beta1 subunits. J. Cell. Sci. 2016-05-03 [PMID: 27142834] (WB, Human)	WB	Human
Tokhtaeva E, Capri J, Marcus EA et al. Septin dynamics are essential for exocytosis J. Biol. Chem. 2015-01-09 [PMID: 25575596] (WB, Human)	WB	Human
Carmosino Monica, Torretta Silvia, Procino Giuseppe et al. Na+/K+-ATPase beta1-subunit is recruited in Na-K-2Cl co-transporter isoform 2 multiprotein complexes in rat kidneys: possible role in blood pressure regulation. J Hypertens. 2014-09-01 [PMID: 24927069] (Rat)		Rat
Gong H, Sun J, Xue W et al. Protective effect of truncated Na+/K+-ATPase beta on ischemia/reperfusion-induced renal injury in rats. Exp. Biol. Med. (Maywood) 2014-04-15 [PMID: 24737734] (WB, Human)	WB	Human
Tokhtaeva E, Sachs G, Sun H et al. Identification of the amino acid region involved in the intercellular interaction between the beta 1 subunits of Na/K-ATPase J Cell Sci 2012-03-15 [PMID: 22328500] (WB, Rat, Canine)	WB	Rat, Canine
Tang WH, Stitham J, Gleim S et al. Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane. J Clin Invest 2011-11-01 [PMID: 22005299] (Human)		Human
Mastrodonato M, Calamita G, Rossi R et al. Expression of H+,K+-ATPase Glycopattern Analysis in the Gastric Gls of Rana esculenta. J Histochem Cytochem :jhc2008.952234. 2008-01-01 [PMID: 19001639] (WB, IHC-P, Xenopus)	WB, IHC-P	Xenopus
Winterstein, C, Trotter, J et al. Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling. J Cell Sci;121(Pt 6):834-42. 2008-03-15 [PMID: 18303048] (WB, Mouse)	WB	Mouse
Pessach, Itai et al. The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation. J Leukoc Biol;80(3):630-9. 2006-09-01 [PMID: 16844764] (WB, Human)	WB	Human
Vagin, O et al. Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells. J Biol Chem;280(52):43159-67. 2005-12-30 [PMID: 16230337] (WB, Human, Canine, Porcine)	WB	Human, Canine, Porcine
Vagin, O et al. Use of the H,K-ATPase beta subunit to identify multiple sorting pathways for plasma membrane delivery in polarized cells. J Biol Chem;280(15):14741-54. 2005-04-15 [PMID: 15695513] (WB, Canine)	WB	Canine
Vagin, O et al. The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting. J Biol Chem;279(37):39026-34. 2004-09-10 [PMID: 15247221] (WB, Porcine)	WB	Porcine
Show All 16 Publications. Collapse Publications.

Array NB300-147

• Sodium Potassium ATPase Beta 1 Antibodies
• Sodium Potassium ATPase Beta 1 Lysate
• Sodium Potassium ATPase Beta 1 Proteins