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SOD2/Mn-SOD Antibody (582)

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Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].
Genetic Strategies: Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted ...read more
Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell ...read more
Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000
Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pm, RM, ShSpecies Glossary
Applications WB
Clone
582
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

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SOD2/Mn-SOD Antibody (582) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SOD2/Mn-SOD. The exact sequence is proprietary.
Predicted Species
Porcine (94%), Chimpanzee (100%), Bovine (94%), Rhesus Macaque (98%), Chicken (91%), Sheep (94%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOD2
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for SOD2/Mn-SOD Antibody (582)

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SOD2