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SGK1 Antibody (4D7-G3)

Images

 
Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. Antibody concentration 5 ug/ml.
Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Western Blot analysis of SGK expression in A-549 ( Cat # L025V1 ).
Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi ( Cat # H00006446-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
ELISA: SGK1 Antibody (4D7-G3) [H00006446-M01] - SGK Antibody (4D7-G3) [H00006446-M01] - Detection limit for recombinant GST tagged SGK is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, KD
Clone
4D7-G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SGK1 Antibody (4D7-G3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SGK (AAH01263.1, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL
Localization
Cytoplasmic and Nuclear (on phosphorylation).
Specificity
SGK - serum/glucocorticoid regulated kinase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SGK1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recominant protein for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SGK1 Antibody (4D7-G3)

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine protein kinase SGK
  • serine/threonine-protein kinase Sgk1
  • serum/glucocorticoid regulated kinase 1
  • serum/glucocorticoid regulated kinase
  • Serum/glucocorticoid-regulated kinase 1
  • SGK
  • SGK1

Background

This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SGK1
Entrez
OMIM
Uniprot