Novus Biologicals products are now on bio-techne.com

Recombinant Mouse FGF basic/FGF2/bFGF Protein

Images

 
Recombinant Mouse FGF basic/FGF2/bFGF (Catalog # 3139-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.3-1.8 ng/mL.
1 μg/lane of Recombinant Mouse FGF basic/FGF2/bFGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse FGF basic/FGF2/bFGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-1.8 ng/mL.
Source
E. coli-derived mouse FGF basic/FGF2/bFGF protein
Ala11-Ser154
Accession #
N-terminal Sequence
Ala11
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
3139-FB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF basic/FGF2/bFGF Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells (5). The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
  3. Presta, M. et al. (2005) Cytokine. Growth Factor Rev. 16:159.
  4. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
  5. Quarto, N. et al. (2005) Gene 356:49.
  6. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Comm. 335:1239.
  7. Hebert, J. M. et al. (1990) Dev. Biol. 138:454.

Customers Who Viewed This Item Also Viewed...

DVE00
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
AF232
Species: Hu
Applications: IHC, Neut, Simple Western, WB
291-G1
Species: Hu
Applications: BA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
H00002263-M01
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
294-HG
Species: Hu
Applications: BA
DBD00
Species: Hu
Applications: ELISA
251-KG
Species: Hu
Applications: BA
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
267-N3
Species: Hu
Applications: BA
256-GF
Species: Hu
Applications: BA

Publications for FGF basic/FGF2/bFGF (3139-FB)(193)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenopus.

We have publications tested in 5 applications: Bioassay, Cell Culture, IP, In Vivo, Stimulation.


Filter By Application
Bioassay
(160)
Cell Culture
(13)
IP
(1)
In Vivo
(19)
Stimulation
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(188)
Rat
(2)
Transgenic Mouse
(3)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 193. Show All 193 Publications.
Publications using 3139-FB Applications Species
Chaube, B;Citrin, KM;Sahraei, M;Singh, AK;de Urturi, DS;Ding, W;Pierce, RW;Raaisa, R;Cardone, R;Kibbey, R;Fernández-Hernando, C;Suárez, Y; Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis Nature communications 2023-12-12 [PMID: 38086791] (Bioassay, Mouse) Bioassay Mouse
Shao, A;Kissil, JL;Fan, CM; The L27 Domain of MPP7 enhances TAZ-YY1 Cooperation to Renew Muscle Stem Cells bioRxiv : the preprint server for biology 2023-11-04 [PMID: 37961392] (Cell Culture, Mouse) Cell Culture Mouse
da Silva, VA;Bobotis, BC;Correia, FF;Lima-Vasconcellos, TH;Chiarantin, GMD;De La Vega, L;Lombello, CB;Willerth, SM;Malmonge, SM;Paschon, V;Kihara, AH; The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration International journal of molecular sciences 2023-09-04 [PMID: 37686446] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Zeng, C;Chen, J;Cooke, EW;Subuddhi, A;Roodman, ET;Chen, FX;Cao, K; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition Nature communications 2023-08-22 [PMID: 37607921] (Bioassay, Mouse) Bioassay Mouse
Zeng, C;Chen, J;Cooke, EW;Subuddhi, A;Roodman, ET;Chen, FX;Cao, K; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition Nature communications 2023-08-22 [PMID: 37607921] (Bioassay, Mouse) Bioassay Mouse
Scacchetti, A;Shields, EJ;Trigg, NA;Wilusz, JE;Conine, CC;Bonasio, R; A ligation-independent sequencing method reveals tRNA-derived RNAs with blocked 3' termini bioRxiv : the preprint server for biology 2023-06-08 [PMID: 37333231] (Bioassay, Mouse) Bioassay Mouse
DuCote, TJ;Song, X;Naughton, KJ;Chen, F;Plaugher, DR;Childress, AR;Edgin, AR;Qu, X;Liu, J;Liu, J;Li, F;Wong, KK;Brainson, CF; EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas bioRxiv : the preprint server for biology 2023-06-08 [PMID: 37333199] (Bioassay, Mouse) Bioassay Mouse
Basu, R;Ganesan, S;Winkler, CW;Anzick, SL;Martens, C;Peterson, KE;Fraser, IDC; Identification of age-specific gene regulators of La Crosse virus neuroinvasion and pathogenesis Nature communications 2023-05-18 [PMID: 37202395] (Bioassay, Mouse) Bioassay Mouse
Pascual, F;Icyuz, M;Karmaus, P;Brooks, A;Van Gorder, E;Fessler, MB;Shaw, ND; Cholesterol biosynthesis modulates differentiation in murine cranial neural crest cells Scientific reports 2023-05-01 [PMID: 37127649] (Bioassay, Mouse) Bioassay Mouse
X Wang, W Fan, N Li, Y Ma, M Yao, G Wang, S He, W Li, J Tan, Q Lu, S Hou YY1 lactylation in microglia promotes angiogenesis through transcription activation-mediated upregulation of FGF2 Genome Biology, 2023-04-21;24(1):87. 2023-04-21 [PMID: 37085894] (Bioassay, Mouse) Bioassay Mouse
Show All 193 Publications.

Reviews for FGF basic/FGF2/bFGF (3139-FB) (0)

There are no reviews for FGF basic/FGF2/bFGF (3139-FB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF basic/FGF2/bFGF (3139-FB). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Additional FGF basic/FGF2/bFGF Products

Blogs on FGF basic/FGF2/bFGF

There are no specific blogs for FGF basic/FGF2/bFGF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse FGF basic/FGF2/bFGF Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Fgf2
Uniprot