Reactivity | MuSpecies Glossary |
Applications | Binding Activity, Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 µg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). Measured by its ability to bind Recombinant Mouse 4‑1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B) in a functional ELISA. |
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Source | Mouse myeloma cell line, NS0-derived mouse 4-1BB Ligand/TNFSF9 protein
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Accession # | |||||||||
N-terminal Sequence | Met |
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Protein/Peptide Type | Recombinant Proteins |
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Gene | Tnfsf9 |
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Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 25.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 41-44 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
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