Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Chicken.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA Capture, SDS-Page.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using 1314-SH	Applications	Species
Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M; Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells Nature communications 2023-12-05 [PMID: 38052784] (Bioassay, Human)	Bioassay	Human
Lee, J;Kim, Y;Ataliotis, P;Kim, HG;Kim, DW;Bennett, DC;Brown, NA;Layman, LC;Kim, SH; Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development Scientific reports 2023-07-29 [PMID: 37516749] (Bioassay, Mouse)	Bioassay	Mouse
J Sun, DY Shin, M Eiseman, AR Yallowitz, N Li, S Lalani, Z Li, M Cung, S Bok, S Debnath, SJ Marquez, TE White, AG Khan, IC Lorenz, JH Shim, FS Lee, R Xu, MB Greenblatt SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts Nature Communications, 2021-07-29;12(1):4611. 2021-07-29 [PMID: 34326333] (ELISA Capture, Human)	ELISA Capture	Human
Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-04-24;11(1):1994. 2020-04-24 [PMID: 32332736] (Bioassay, Mouse)	Bioassay	Mouse
Y Arribat, KS Mysiak, L Lescouzère, A Boizot, M Ruiz, M Rossel, P Bomont Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy J. Clin. Invest., 2019-12-02;0(0):. 2019-12-02 [PMID: 31503551] (Cell Culture, Mouse)	Cell Culture	Mouse
T Johansen, C Krabbe, SI Schmidt, AM Serrano, M Meyer Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells Stem Cells Int, 2017-10-22;2017(0):9605432. 2017-10-22 [PMID: 29201062] (Bioassay, Human)	Bioassay	Human
E El Agha, V Kheirollah, A Moiseenko, W Seeger, S Bellusci Ex vivo analysis of the contribution of FGF10(+) cells to airway smooth muscle cell formation during early lung development Dev. Dyn., 2017-06-01;0(0):. 2017-06-01 [PMID: 28387977] (Bioassay, Mouse)	Bioassay	Mouse
Dye , Briana R, Hill , David R, Ferguson , Michael, Tsai , Yu-Hwai, Nagy , Melinda, Dyal , Rachel, Wells , James M, Mayhew , Christop, Nattiv , Roy, Klein , Ophir D, White , Eric S, Deutsch , Gail H, Spence , Jason R In vitro generation of human pluripotent stem cell derived lung organoids. Elife, 2015-03-24;4(0):. 2015-03-24 [PMID: 25803487] (Bioassay, Human)	Bioassay	Human
Xu Y, An Y, Wang X, Zha W, Li X Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep, 2013-11-28;31(2):707-12. 2013-11-28 [PMID: 24297612] (Bioassay, Human)	Bioassay	Human
Mills , Lisa D, Zhang , Yaqing, Marler , Ronald J, Herreros-Villanueva , Marta, Zhang , Lizhi, Almada , Luciana, Couch , Fergus, Wetmore , Cynthia, Pasca di Magliano , Marina, Fernandez-Zapico , Martin E Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation. J Biol Chem, 2013-03-12;288(17):11786-94. 2013-03-12 [PMID: 23482563] (Bioassay, Mouse)	Bioassay	Mouse
Aad , Pauline, Echternkamp , Sherrill, Sypherd , David D, Schreiber , Nicole B, Spicer , Leon J The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 2012-10-04;87(4):79. 2012-10-04 [PMID: 22811575] (Bioassay, Bovine)	Bioassay	Bovine
Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012-03-01;20(0):1H.51. 2012-03-01 [PMID: 22415839] (Bioassay, Human)	Bioassay	Human
Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009-01-20;583(3):561-7. 2009-01-20 [PMID: 19162023] (Bioassay, Human)	Bioassay	Human
Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 2008-01-15;22(2):152-65. 2008-01-15 [PMID: 18198334] (Bioassay, Human)	Bioassay	Human
Zavros Y, Waghray M, Tessier A, Bai L, Todisco A, L Gumucio D, Samuelson LC, Dlugosz A, Merchant JL Reduced pepsin A processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation. J. Biol. Chem., 2007-09-14;282(46):33265-74. 2007-09-14 [PMID: 17872943] (SDS-Page)	SDS-Page
Nordstrom U, Maier E, Jessell TM, Edlund T An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity. PLoS Biol., 2006-07-01;4(8):e252. 2006-07-01 [PMID: 16895440] (Bioassay, Chicken)	Bioassay	Chicken
Show All 16 Publications. Collapse Publications.

Array 1314-SH

• Sonic Hedgehog/Shh Antibodies
• Sonic Hedgehog/Shh ELISA Kits
• Sonic Hedgehog/Shh Lysate
• Sonic Hedgehog/Shh Proteins
• Sonic Hedgehog/Shh Proteins and Enzymes
• Sonic Hedgehog/Shh ELISAs