Recombinant Human MAdCAM-1 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins (1). Human MAdCAM-1 is synthesized as a 382 amino acid (aa) precursor that contains an 18 aa signal sequence, a 299 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there is one potential site for N-linked glycosylation (2). The ECD comprises two Ig-like domains of 90 aa and 119 aa, respectively, each possessing invariant cysteine residues that stabilize the Ig loop (2). There is also a Ser-Thr-Pro-rich (71%) mucin-like 48 aa domain that is (aa 206 ‑ 317) formed by six tandem repeats of an eight aa sequence having the general consensus DTTSPEP/SP. This mucin domain contains 19 potential sites for O-linked glycosylation (2, 3). A splicing variant in which a single Ala residue is substituted for aa 223 ‑ 334 in isoform 1 produces a second isoform. Human mature MAdCAM-1 shares only 44% aa sequence identity with mature mouse MAdCAM-1. The integrin alpha (4)  beta (7), which is expressed on lymphocytes, functions as the MAdCAM-1 receptor (1). The Ig domains of MAdCAM-1 are critical to alpha (4)  beta (7)  binding, and the mucin domain has activity in L‑Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha  and IL‑1 beta . MAdCAM-1 is expressed on the surface of high endothelial venules (HEV) in the gut and in Peyer’s patches, on endothelial cells of the mesenteric lymph nodes, lamina propria of the small and large intestine, and the mammary gland during lactation, and on brain endothelial cells (1). MAdCAM‑1 has also been reported to be expressed in the liver portal region in autoimmune hepatitis (1), and in bone marrow following allogenic (genetically non-identical) hematopoietic stem cell transplantation, where it recruits donor T cells, which may lead to graft versus host disease (3, 4). MAdCAM‑1 functions as a homing receptor, and plays a central role in leukocyte migration into HEVs and Peyer’s patch (5). In addition to its normal role in lymphocyte trafficking to mucosal tissue, MAdCAM‑1 expression is also dramatically increased in chronic inflammatory and disease states (1, 6), including inflammatory bowel disease (Crohn’s disease and ulcerative colitis) (7), sclerosing cholangitis (8), and diabetes (9), and may play an important role in these conditions.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA Capture Control, Flow Cytometry, Surface Plasmon Resonance.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using 6056-MC	Applications	Species
Su, Y;Luo, Z;Sun, D;Yang, B;Li, Q; The Force-Dependent Mechanism of an Integrin ?4?7-MAdCAM-1 Interaction International journal of molecular sciences 2023-11-07 [PMID: 38003252] (Bioassay, Human)	Bioassay	Human
Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M; E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation Nature immunology 2023-10-30 [PMID: 37903858] (Bioassay, Human)	Bioassay	Human
Sun, D;Luo, Z;Kong, Y;Huang, R;Li, Q; Force-Regulated Calcium Signaling of Lymphoid Cell RPMI 8226 Mediated by Integrin ?4?7/MAdCAM-1 in Flow Biomolecules 2023-03-24 [PMID: 37189336] (Bioassay, Human)	Bioassay	Human
PK Myint, A Ito, MG Appiah, G Obeng, S Darkwah, E Kawamoto, A Gaowa, EJ Park, M Shimaoka Irisin supports integrin-mediated cell adhesion of lymphocytes Biochemistry and Biophysics Reports, 2021-03-08;26(0):100977. 2021-03-08 [PMID: 33732908] (Bioassay, ELISA Capture Control, Human)	Bioassay, ELISA Capture Control	Human
R Domenis, M Marino, A Cifù, G Scardino, F Curcio, M Fabris Circulating exosomes express &alpha4&beta7 integrin and compete with CD4+ T cells for the binding to Vedolizumab PLoS ONE, 2020-11-12;15(11):e0242342. 2020-11-12 [PMID: 33180848] (Bioassay, Human)	Bioassay	Human
C Allner, M Melde, E Becker, F Fuchs, L Mühl, E Klenske, L Müller, N Morgenster, K Fietkau, S Hirschmann, R Atreya, I Atreya, MF Neurath, S Zundler Baseline levels of dynamic CD4+ T cell adhesion to MAdCAM-1 correlate with clinical response to vedolizumab treatment in ulcerative colitis: a cohort study BMC Gastroenterol, 2020-04-15;20(1):103. 2020-04-15 [PMID: 32293299] (Bioassay, Human)	Bioassay	Human
A Natoni, ML Farrell, S Harris, C Falank, L Kirkham-Mc, MS Macauley, MR Reagan, M O' Dwyer Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model Haematologica, 2020-01-31;0(0):. 2020-01-31 [PMID: 31101754] (Bioassay, Human)	Bioassay	Human
C Lichnog, S Klabunde, E Becker, F Fuh, P Tripal, R Atreya, E Klenske, R Erickson, H Chiu, C Reed, S Chung, C Neufert, I Atreya, J McBride, MF Neurath, S Zundler Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease Front Pharmacol, 2019-02-01;10(0):39. 2019-02-01 [PMID: 30774593] (Bioassay, Human)	Bioassay	Human
S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7 PLoS Pathog., 2018-08-28;14(8):e1007278. 2018-08-28 [PMID: 30153309] (Bioassay, Surface Plasmon Resonance, Human)	Bioassay, Surface Plasmon Resonance	Human
KW Cheung, T Wu, SF Ho, YC Wong, L Liu, H Wang, Z Chen ??CD4effector/effector memory T cells differentiate into productively and latently infected central memory T cells by TGF?1 during HIV-1 infection J. Virol., 2018-03-28;0(0):. 2018-03-28 [PMID: 29386290] (Flow Cytometry, Human)	Flow Cytometry	Human
L Wang, SS DeMarco, MS Peaks, AL Maiorana-B, J Chen, MJ Crouch, BM Shewchuk, SR Shaikh, CM Phillips, LC Bridges RAR?/RXR Synergism Potentiates Retinoid Responsiveness in Cutaneous T Cell Lymphoma Cell Lines Exp. Dermatol., 2017-07-03;0(0):. 2017-07-03 [PMID: 28370539] (Bioassay, Human)	Bioassay	Human
Peachman K, Karasavvas N, Chenine A, McLinden R, Rerks-Ngarm S, Jaranit K, Nitayaphan S, Pitisuttithum P, Tovanabutra S, Zolla-Pazner S, Michael N, Kim J, Alving C, Rao M Identification of New Regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to alpha4beta7 Integrin Receptor. PLoS ONE, 2015-12-01;10(12):e0143895. 2015-12-01 [PMID: 26625359] (Bioassay, Human)	Bioassay	Human
Perez L, Chen H, Liao H, Montefiori D Envelope glycoprotein binding to the integrin alpha4beta7 is not a general property of most HIV-1 strains. J Virol, 2014-07-09;88(18):10767-77. 2014-07-09 [PMID: 25008916] (Bioassay, Human)	Bioassay	Human
Li H, Pauza CD The alpha4beta7 integrin binds HIV envelope but does not mediate bystander killing of gamma/delta T cells. Blood, 2012-07-19;120(3):698-9. 2012-07-19 [PMID: 22822002] (Bioassay, Human)	Bioassay	Human
Li H, Pauza CD HIV envelope-mediated, CCR5/alpha4beta7-dependent killing of CD4-negative gammadelta T cells which are lost during progression to AIDS. Blood, 2011-09-16;118(22):5824-31. 2011-09-16 [PMID: 21926353] (Binding Assay, Human)	Binding Assay	Human
Show All 15 Publications. Collapse Publications.

Array 6056-MC

• MAdCAM-1 Antibodies
• MAdCAM-1 Lysates
• MAdCAM-1 Protein
• MAdCAM-1 Proteins and Enzymes
• MAdCAM-1 ELISAs