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Recombinant Human IL-19 (NS0-expressed) Protein, CF

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Recombinant Human IL-19 (Catalog # 9286-IL/CF) induces proliferation of BaF3 mouse pro-B cells transfected with humanIL-20 R alpha and humanIL-20 R beta . The ED50 for this effect is 0.1-0.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-19 (NS0-expressed) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta . The ED50 for this effect is 0.1-0.6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-19 protein
Leu25-Ala177
Accession #
N-terminal Sequence
Leu25
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-30 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-19 (NS0-expressed) Protein, CF

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19

Background

Interleukin 19 (IL-19) is a member of the IL-10 family of class II alpha -helical cytokines that contains two groups, a viral homolog and cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R beta as a signal transducing receptor (IL-10, IL-22, and IL-26), and one which uses IL-20 R beta as a signal transducing receptor (IL-19, IL-20, and IL-24) (1). The 153 amino acid (aa) mature human IL-19 is secreted as a glycosylated 35-45 kDa monomer (2-4). It shares approximately 70% aa sequence identity with mouse and rat IL-19. Human IL-19 may utilize an alternate start site that adds 38 aa to the N-terminus. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (2). IL-19 is expressed on activated keratinocytes, monocytes, and B cells (3, 5, 6). It binds a receptor complex consisting of IL-20 R alpha and IL-20 R beta (3, 7, 8). This receptor complex is also shared by IL-20 and IL-24 (9, 10). It has been reported that IL-19 both will and will not induce IL-6 and TNF-alpha production by monocytes (2, 11). It does, however, drive T helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (2, 11, 12).
  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Liao, Y-C. et al. (2002) J. Immunol. 169:4288.
  3. Gallagher, G. et al. (2000) Genes Immun. 1:442.
  4. Pletnev, S. et al. (2003) Biochemistry 42:12617.
  5. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  6. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  7. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  8. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
  9. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  10. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  11. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  12. Liao, S-C. et al. (2004) J. Immunol. 173:6712.

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