Recombinant Human Furin His-tag Protein, CF Summary
Details of Functionality |
Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (Catalog # <a class=NoLineLink href='http://www.rndsystems.com/product_results.aspx?k=ES013'>ES013</a>).<br />The specific activity is >125 pmol/min/μg, as measured under the described conditions.<br /><br /> |
Accession # |
|
N-terminal Sequence |
Asp108 |
Structure / Form |
Mature |
Protein/Peptide Type |
Recombinant Enzymes |
Endotoxin Note |
<0.100 EU per 1 µg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
44-66 kDa, under reducing conditions |
Packaging, Storage & Formulations
Storage |
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Buffer |
Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl, Brij-35 and Glycerol. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Furin His-tag Protein, CF
Background
Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease (1-3). As a cellular protease, Furin cleaves after Arg-Xaa-Lys/Arg-Arg-like motifs typically at the end of the pro region in a variety of proproteins within the secretory pathway including growth factors and receptors, extracellular matrix proteins, and other proteases. Through regulation of proprotein maturation, Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer (4), neurodegenerative diseases (5) and infectious disease (6). Furin is synthesized as a 794 amino acid type I transmembrane protein precursor with a signal peptide (residues 1-24), a pro region (residues 25-107) that plays a crucial role in the folding, activation and transport of Furin, and a mature chain (residues 108-794) (1-3). The mature chain consists of the subtilisin-like catalytic domain, a P domain, which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain, which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin corresponds to a truncated form of the mature enzyme terminated before the transmembrane domain.
- Van den Ouweland, A.M. et al. (1990) Nucleic Acids Res. 18:664.
- Barr, P.J. et al. (1991) DNA Cell Biol. 10:319.
- Thomas, G. (2002) Nature Rev. Mol. Cell Biol. 3:753.
- He, Z. et al. (2022) Oncogene. 9:1252.
- Zhang, Y. et al. (2022) Transl. Neurodegener. 11:39.
- Braun, E. and D. Sauter (2019) Clin. Transl. Immunology. 8:e1073.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu
Applications: ICC, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Ch, Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Hu
Applications: BA
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
Species: Hu
Applications: IHC, IHC-P
Species: Ca(-), Hu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, IF, IHC, IHC-P, MI, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Publications for Furin (11338-SE) (0)
There are no publications for Furin (11338-SE).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Furin (11338-SE) (0)
There are no reviews for Furin (11338-SE).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for Furin (11338-SE) (0)
Additional Furin Products
Blogs on Furin