Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. When Recombinant Human SCF (Mammalian-expressed,
Catalog #
7644-SC)
is coated at 1 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag binds with an ED 50 of 0.05-0.30 μg/mL. |
Source |
Human embryonic kidney cell, HEK293-derived human CD117/c-kit protein Human CD117/c-kit (Gln26-Thr520) Accession # P10721.1 | IEGRMD | Human IgG1 (Pro100-Lys330) | Avi-tag | N-terminus | | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
Gln26 (Blocked) |
Structure / Form |
Disulfide-linked homodimer, biotinylated via Avi-tag |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
100-120 kDa, under reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF
Background
Stem cell factor receptor (SCF R), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that
acts as the receptor for the cytokine SCF (1, 2). Mature human SCF R consists of an
extracellular domain (ECD) with five tandem immunoglobulin-like
domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain.
Within the ECD, human SCF R shares 73% and 76%
amino acid sequence identity with mouse and rat SCF R, respectively. At
least 4 SCF R isoforms, produced by alternative mRNA splicing, have been
identified in humans and 2 are characterized by the presence or absence of a
tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (3, 4). Human
SCF R is expressed in embryonic stem cells and is involved in regulation of
cell survival and proliferation, hematopoiesis, gametogenesis, and
melanogenesis (1, 2, 5, 6). SCF R interacts with noncovalent dimers of
transmembrane or soluble SCF to trigger receptor dimerization and initiate signaling
(7). Our Avi-tag Biotinylated rhSCF R features biotinylation at a single site
contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when
bound to streptavidin-coated surface due to the precise control of
biotinylation and the rest of the protein is unchanged so there is no
interference in the protein's bioactivity.
- Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
- Ashman, L.K. et al. (1999) Inter Jour of Biochem & Cell Bio. 31:1037.
- Lennartsson J. and Rönnstrand L. (2012) Physiol Rev. 92:1619
- Caruana, G. et al. (1999) Oncogene 18:5573
- Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
- Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
- Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: BA
Species: Rt
Applications: IHC, WB
Species: Mu
Applications: Flow, IHC, Neut, WB
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: ICC, IHC
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu
Applications: CyTOF-ready, Flow
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu
Applications: IHC
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: BA
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: BA
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
Publications for CD117/c-kit (AVI10703) (0)
There are no publications for CD117/c-kit (AVI10703).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for CD117/c-kit (AVI10703) (0)
There are no reviews for CD117/c-kit (AVI10703).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for CD117/c-kit (AVI10703) (0)