Home » SGK1 » SGK1 Proteins » Recombinant Human Active SGK1 (60-end) Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human Active SGK1 (60-end) Protein, CF

 Submit a Review

Images

 
(
Enlarge)
The approximate molecular weight is 73 kDa and the average purity is 95%.
The approximate molecular weight is 73 kDa and the average purity is 95%.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human Active SGK1 (60-end) Protein, CF Summary

Details of Functionality
The specific activity of SGK1 was determined to be 99 nmol/min/mg using a Akt (PKB) peptide substrate as per activity assay protocol.
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human SGK1 protein
aa 60-431
With an N-terminal GST tag
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
SGK1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Applications/Dilutions

Dilutions
  • Bioactivity
SDS-PAGE
73 kDa

Packaging, Storage & Formulations

Storage
This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Buffer
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Assay Procedure
  • Active Kinase - Active SGK1 (0.1 μg/μL) diluted with Kinase Dilution Buffer to the concentrations indicated in Figure 2. Note: These are suggested working dilutions. Optimal dilutions should be determined by each laboratory for each application.
  • Kinase Assay Buffer I - 25 mM MOPS, pH 7.2, 12.5 mM beta -glycerol-phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA. Add 0.25 mM DTT to the Kinase Assay Buffer I prior to use.
  • Kinase Dilution Buffer - Kinase Assay Buffer I diluted at a 1:4 ratio (5-fold dilution) with distilled or deionized water.
  • 10 mM ATP Stock Solution - Prepare the ATP Stock Solution by dissolving 55 mg of ATP in 10 mL of Kinase Assay Buffer I. Store 200 μL aliquots at ≤ -20°C.
  • [33P]-ATP Assay Cocktail - Prepare 250 μM [33P]-ATP Assay Cocktail in a designated radioactive work area by combining 150 μL of 10 mM ATP Stock Solution, 100 μL of [33P]-ATP (1 mCi/100 μL), and 5.75 mL of Kinase Assay Buffer I. Store 1.0 mL aliquots at ≤ -20°C.
  • Substrate - Akt (PKB) peptide substrate (CKRPRAASFAE) diluted in distilled or deionized water to a final concentration of 1.0 mg/mL.
  1. Thaw the [33P]-ATP Assay Cocktail in a shielded container in a designated radioactive work area.
  2. Thaw the Active SGK1, Kinase Assay Buffer I, Substrate, and Kinase Dilution Buffer on ice.
  3. In a pre-cooled microfuge tube, add the following reaction components bringing the initial reaction volume up to 20 μL.
    a. Diluted Active SGK1: 10 μL
    b. Substrate (1 mg/mL Stock Solution): 5 μL
    c. Distilled water: 5 μL
  4. Set up the blank control as outlined in step 3, excluding the addition of the substrate. Replace the substrate with an equal volume of distilled water.
  5. Initiate the reaction by the addition of 5 μL [33P]-ATP Assay Cocktail, bringing the final volume up to 25 μL. Incubate the mixture in a water bath at 30 °C for 15 minutes.
  6. After the 15 minute incubation period, terminate the reaction by spotting 20 μL of the reaction mixture onto individual pre-cut strips of phospho cellulose P81 paper.
  7. Air dry the pre-cut P81 strip and sequentially wash in a 1% phosphoric acid solution (add 10 mL of phosphoric acid to 990 mL deionized water) with constant gentle stirring. It is recommended that the strips be washed a total of three times for approximately 10 minutes each.
  8. Count the radioactivity on the P81 paper in the presence of scintillation fluid in a scintillation counter.
  9. Determine the corrected cpm by removing the blank control value (see step 4) for each sample and calculate the kinase specific activity as outlined below:


    Calculation of [33P]-ATP Specific Activity (SA) (cpm/pmol)
    Specific Activity (SA) = cpm for 5 μL [33P]-ATP/pmol of ATP (in 5 μL of a 250 μM ATP stock solution; i.e. 1250 pmol)

    Calculation of Kinase Specific Activity (SA) (pmol/minutes/μg or nmol/minutes/mg)
    Corrected cpm from reaction / [(SA of 33P-ATP in cpm/pmol) x (Reaction time in minutes) x (Enzyme amount in μg or mg)] x [(Reaction volume) / (Spot Volume)]

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Active SGK1 (60-end) Protein, CF

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine protein kinase SGK
  • serine/threonine-protein kinase Sgk1
  • serum/glucocorticoid regulated kinase 1
  • serum/glucocorticoid regulated kinase
  • Serum/glucocorticoid-regulated kinase 1
  • SGK
  • SGK1

Background

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK 1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1 (2). SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.

  1. Kobayashii, T. et al. (1999) Biochem. J. 1:189.
  2. Brunet, A. et al. (2001) Mol. Cell. Biol. 21:952.

Customers Who Viewed This Item Also Viewed...

AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
PYK2/FAK2 [p
Tyr402] Antibody (592 ...
MAB6210
Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]
Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: Simple Western, WB
SGK3 Antibody
NBP3-12271
Immunohistochemistry-Paraffin SGK3 Antibody
Immunohistochemistry-Paraffin SGK3 Antibody
Immunohistochemistry-Paraffin SGK3 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Recombinant Human
NEDD4L GST (N-Term) P ...
H00023327-P01
Other Recombinant Human NEDD4L GST (N-Term) Protein
(3 Publications)
Species: Hu
Applications: ELISA, AP, PA, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
PDK-1 Antibody
(650308) [Unconjugate
MAB864
Immunocytochemistry PDK-1 Antibody (650308) [Unconjugated]
Western Blot PDK-1 Antibody (650308) [Unconjugated]
Simple Western PDK-1 Antibody (650308) [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
Pyruvate
Dehydrogenase Kinase ...
NB100-2383
Immunocytochemistry/ Immunofluorescence Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Western Blot Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
Western Blot Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody
(2 Reviews)
(6 Publications)
Species: Hu
Applications: ICC/IF, WB
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody
Western Blot TOR/mTOR [p Ser2448] Antibody
Data TOR/mTOR [p Ser2448] Antibody
(2 Reviews)
(9 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
GR/NR3C1 Antibody
(BuGR2) - BSA Free
NB300-731
Flow Cytometry GR/NR3C1 Antibody (BuGR2) - BSA Free
Flow Cytometry GR/NR3C1 Antibody (BuGR2) - BSA Free
Immunocytochemistry/ Immunofluorescence GR/NR3C1 Antibody (BuGR2) - BSA Free
(3 Reviews)
(12 Publications)
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
Mineralocorticoid
R/NR3C2 Antibody (H10 ...
NB300-562
Immunohistochemistry-Paraffin Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) - BSA Free
Western Blot Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) - BSA Free
Immunocytochemistry/ Immunofluorescence Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) - BSA Free
(2 Publications)
Species: Ch, Hu, Mu, Rb, Rt, Sh
Applications: B/N, Flow, ICC/IF, IHC, IHC-P, WB
SGK2 Antibody
NBP1-86102
Western Blot SGK2 Antibody
Immunohistochemistry-Paraffin SGK2 Antibody
Immunohistochemistry-Paraffin SGK2 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PIK3CA Antibody
NBP2-19804
Western Blot PIK3CA Antibody
Immunohistochemistry-Paraffin PIK3CA Antibody
Western Blot PIK3CA Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
beta/PIK3CB Antibody ...
MAB2686
Western Blot PI 3-Kinase p110 beta/PIK3CB Antibody (269020)
(5 Publications)
Species: Hu
Applications: WB
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot PI 3-Kinase p110 gamma Antibody
Immunohistochemistry-Paraffin PI 3-Kinase p110 gamma Antibody
Western Blot PI 3-Kinase p110 gamma Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
PI 3-Kinase p110
delta Antibody (OTI1D ...
NBP2-46404
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
Immunohistochemistry PI 3-Kinase p110 delta Antibody (OTI1D11)
(1 Review)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
KCNJ1 Antibody
NBP1-82874
Immunohistochemistry-Paraffin KCNJ1 Antibody
Immunohistochemistry-Paraffin KCNJ1 Antibody
Immunohistochemistry-Paraffin KCNJ1 Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
p70 S6 Kinase/S6K
[p Thr389/412] Antibo ...
NB600-1049
Immunocytochemistry/ Immunofluorescence p70 S6 Kinase/S6K [p Thr389/412] Antibody
Immunohistochemistry p70 S6 Kinase/S6K [p Thr389/412] Antibody
Western Blot p70 S6 Kinase/S6K [p Thr389/412] Antibody
(1 Review)
(7 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
FOXO3 Antibody
NBP2-16521
Western Blot FOXO3 Antibody
Western Blot FOXO3 Antibody
Immunocytochemistry/ Immunofluorescence FOXO3 Antibody
(4 Reviews)
(12 Publications)
Species: Hu, Mu, Rt, Sq, Xp
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, KD, WB

Publications for SGK1 (3200-KS) (0)

There are no publications for SGK1 (3200-KS).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SGK1 (3200-KS) (0)

There are no reviews for SGK1 (3200-KS). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for SGK1 (3200-KS) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SGK1 Products

Blogs on SGK1

There are no specific blogs for SGK1, but you can read our latest blog posts.

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Active SGK1 (60-end) Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol SGK1
Uniprot