Reactivity | HuSpecies Glossary |
Applications | Binding Activity |
Format | Carrier-Free |
Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B) is Immobilized at 10 ng/mL (100 µL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5‑2.5 ng/mL. |
Source | E. coli-derived human 4-1BB Ligand/TNFSF9 protein Arg71-Glu254, with an N-terminal Met and a 6-His tag |
Accession # | |
N-terminal Sequence | Met |
Protein/Peptide Type | Recombinant Proteins |
Gene | TNFSF9 |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 20.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose. |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
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