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RALBP1 Antibody (OTI11B2) - Azide and BSA Free

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Western Blot: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded prostate tissue using anti-RALBP1 mouse monoclonal antibody.
Flow Cytometry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Analysis of Jurkat cells, using anti-RALBP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded breast tissue using anti-RALBP1 mouse monoclonal antibody.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-RALBP1 mouse monoclonal antibody.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-RALBP1 mouse monoclonal antibody.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded endometrium tissue using anti-RALBP1 mouse monoclonal antibody.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded Ovary tissue using anti-RALBP1 mouse monoclonal antibody.
Immunoprecipitation: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
OTI11B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

RALBP1 Antibody (OTI11B2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RALBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation 4ug/ml
  • Western Blot 1:2000
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for RALBP1 Antibody (OTI11B2) - Azide and BSA Free

  • Dinitrophenyl S-glutathione ATPase
  • ralA binding protein 1
  • ralA-binding protein 1,76 kDa Ral-interacting protein
  • RalBP1
  • Ral-interacting protein 1
  • RIP
  • RIP1
  • RLIP1
  • RLIP76DNP-SG ATPase

Background

The receptor interacting protein kinase 1 (RIP1) is essential for the activation of nuclear factor kappaB (NF-kappaB) by tumor necrosis factor alpha (TNFalpha). RIP1 can be polyubiquitinated at Lys 377, which is required for the activation of IkappaB kinase (IKK) and NF-kappaB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RALBP1